锌离子存在下EGCG对前列腺癌细胞生长的影响

GROWTH INHIBITION OF PROSTATE CANCER CELLS BY EPIGALLOCATECHIN-3-GALLATE IN THE PRESENCE OF Zn^(2+)IN VITRO

本文研究了锌离子存在下EGCG对前列腺癌细胞PC-3生长的影响.研究发现Zn^(2+)可以增强EGCG抗癌活性,Zn^(2+)存在下,EGCG处理后前列腺癌细胞PC-3克隆形成率显著下降。以RT- PCR、免疫组化方法研究Zn^(2+)、EGCG对67 kD层粘连蛋白受体(67kD Laminin Receptor,67LR)表达调控,结果表明Zn^(2+)可通过上调67LR的表达。为EGCG提供更多作用的靶位点,增强EGCG对前列腺癌细胞PC-3的毒性作用。MMP-9是肿瘤侵袭转移过程中关键的基质金属蛋白酶,MMP- 9活性与癌细胞的转移潜能密切相关。本文研究发现Zn^(2+)、EGCG处理可通过抑制MMP-9活性,降低前列腺癌细胞PC-3的迁移率.其中80μmol/L EGCG+80μmol/L Zn^(2+)处理24h后显著抑制了PC-3细胞的迁移率。

In this paper, the cytotoxicity of EGCG against PC-3 prostate cancer cells and its molecular mechanism in the presence and absence of Zn^2＋ in vitro were investigated. The results showed that both EGCG and Zn^2＋ suppressed clonegenecity of PC-3 cells, and the suppression effect was enhanced in the coexist system of EGCG and Zn^2＋ MMP-9 is thought to play a significant role in cancer cell migration and invasion. In the present paper, the results showed that EGCG suppressed the activity of MMP-9 in PC-3 cells in the presence of Zn^2＋, as a result, migration ability of the cells was significantly decreased.