姜黄素诱导人成骨肉瘤MG-63细胞凋亡过程中核基质蛋白表达的变化

CHANGES OF NUCLEAR MATRIX PROTEINS DURING APOPTOSIS OF HUMAN OSTEOSARCOMA MG-63 CELLS INDUCED BY CURCUMIN

应用选择性抽提、整装透射电镜观察和双向聚丙烯酰胺凝胶电泳与质谱鉴定技术研究细胞凋亡诱导物姜黄素处理前后人成骨肉瘤MG-63细胞核基质-中间纤维系统的构型变化.及其核基质蛋白表达的差异。经姜黄素处理后.MG-63细胞的核基质和中间纤维比对照组明显稀疏,且分布更加不均匀,并分别与核纤层连系,形成趋于断裂但相对还较为完整的网状结构:双向凝胶电泳分析显示在姜黄素诱导MG-63细胞凋亡前后存在27个差异表达的核基质蛋白,经质谱鉴定了其中的21个蛋白.在凋亡的MG-63细胞中表达上调的蛋白鉴定为:DNA聚合酶zeta等7种蛋白:表达下调的蛋白质为:Prohibitin等14种蛋白。其中首次在核基质中发现的蛋白质有17个。因此,在MG-63细胞凋亡过程中不仅其核基质-中间纤维系统构型产生了典型的的凋亡特征性变化.而且伴有核基质蛋白表达的差异。由此证实了与肿瘤细胞凋亡诱导相关特异核基质蛋白的存在及其对肿瘤细胞凋亡诱导的调控作用.从而对揭示核基质构型及其蛋白组成与细胞凋亡的关系和阐明细胞凋亡过程中的基因表达调控机理.均具有重要意义。

Human osteosarcoma MG-63 cells were induced into apoptosis by curcumin （Cur） . Its nuclear matrix proteins were selectively extracted, and subjected to two dimensional gel electrophoresis analysis. The resulted protein patterns were analyzed by Melanie software. There were 27 spots changed remarkably during the apoptosis induced by curcumin, 21 of which were identified. There were seven up-regulated proteins including DNA polymerase zeta and forteen down-regulated proteins including Prohibitin. This study suggests that the induced apoptosis of carcinoma cells is accompanied with changes of nuclear matrix proteins, and confirms the presence of some specific nuclear matrix proteins associated with carcinoma cell growth, apoptosis and the associated signal transduction pathways in induced apoptosis of carcinoma cells. It provides proofs and a new way to study the mechanisms of gene expression carcinogenesis which reveal the relationship between configuration and composition of nuclear matrix and cell apoptosis. Besides, this study provides several potential target proteins for cancer diagnosis and cancer therapy.