APM诱导蒜分生组织细胞染色体结构变异与蛋白质组变化(简报)(英文)

APM INDUCED THE CHROMOSOME STRUCTURE VARIATION AND PROTEOME CHANGE OF ROOT MERISTEMS IN ALLIUM STAIVUM

当蒜根尖分生组织细胞在4μmol/L、6μmol/L、8μmol/L、10μmol/L微管解聚型除草剂APM处理16h后,多极分裂细胞、凝集染色体、桥-断片及微核细胞可明显被观察到,其中多极分裂细胞可分别达10.7%、10.7%、11.1%和10.0%;凝集染色体为5.5%、5.9%、7.1%和9.1%;出现桥和断片的频率为1.9%、1.8%、2.7%和5.5%;出现微核细胞的频率分别为2.8%、8.7%、12.5%和16.3%。分生组织内蛋白质组也产生了明显变化,有5个分子量和等电点分别为66kD/pI6.3、48kD/pI6.6、48kD/pI6.9、16kD/pI5.6、18kD/pI5.2的蛋白质在8μmol/L APM处理的分生组织中被合成,通过MALDI-TOF-MS分析和NCBI 20070528数据库查询,其中3个新合成的蛋白质被确认,它们是S-腺苷甲硫氨酸合成酶、抗坏血酸过氧化物酶和磷酸甘油酸激酶C。染色体结构变异和蛋白质组变化被认为与APM的处理有关的。

Amiprophos methyl （APM） is a kind of phosphoric amide herbicide and directly interrupts microtubule dynamics in plant cell. Many experiments demonstrated that cell division could be blocked at the metaphase in root meristems treated with APM. Therefore, APM has been widely used to induce mitotic metaphase synchronization in order to isolate mass metaphase chromosomes for further analysis of biochemical composition and morphological structure. Although the use of phosphoric amide herbicide has been beneficial to agriculture in China,their improper use is currently becoming a major factor of envi- ronmental pollution, further, the persistence of some of these compounds in plant-derived foods may have a detrimental impact on both animal and human health.