BLU基因慢病毒表达载体的构建

Construction of BLU Gene Recombinant Lentivirus Vector

目的:构建BLU基因慢病毒表达载体。方法:采用PCR技术从人脐带来源的间充质干细胞中扩增出BLU基因,将其接入慢病毒载体(pSL6-IRES-EGFP)中,经菌落PCR、酶切和测序鉴定,然后转染293T细胞观察表达情况。结果:PCR、酶切和测序鉴定克隆了重组子(pSL6-BLU-IRES-EGFP),并在293T细胞中成功表达BLU基因。结论:成功地克隆了含有BLU基因的慢病毒表达载体。

Objective： To construct the lentivims vector containing BLU gene. Methods： The full-length BLU gene was amplified by PCR technique from the total RNA of the umbilical mesenchymal stem cells. BLU gene was subcloned into pSL6-IRES-EGFP vector. The identification was performed by analysis of restricting enzyme digestion and DNA sequence. Results： pSL6-BIu-IRES-EGFP was constructed successfully and identified by PCR, digestion and sequencing. The recombinant could express in 293T cell. Conclusion： The recombinant lentivirus vector containing BLU gene is cloned successfully.