D2-40标记的微淋巴管密度与直肠癌淋巴转移的关系

Relationship between Lymphatic Microvessels Density Marked by Monoclonal Antibody D2-40 and Lymph Node Metastasis in Rectal Carcinoma

目的:研究直肠癌组织中D2-40标记的微淋巴管特点,探讨其与肿瘤淋巴结转移的关系。方法:收集直肠癌和癌旁组织标本90份及正常直肠组织标本30份,用免疫组化SP法检测D2-40表达,计算D2-40标记的微淋巴管密度(LMVD),并结合直肠癌临床病理特征进行相关性分析。结果:肿瘤边缘区LMVD(11.3±4.6)明显高于肿瘤中心区(5.1±2.1)(P<0.01),两者均明显高于正常直肠组织(3.8±2.5)(P<0.01)。直肠癌边缘区高LMVD与肿瘤浸润深度(P<0.05)、淋巴结转移、远处转移和Dukes分期(P<0.01)有关,与患者的性别、年龄、肿瘤大小、部位及分化程度均无关。结论:直肠癌边缘区高LMVD与肿瘤组织的淋巴结转移密切相关。肿瘤边缘区LMVD是判断肿瘤淋巴管生成和淋巴结转移的重要指标。

Objective： To investigate the feature of lymphatic microvessels marked by D2-40 in rectal carcinoma as well as its correlations with the lymph node metastasis, Methods： Ninety tissue specimens of the rectal carcinoma and the peritumoral tissue and thirty specimens of adjacent normal bowel tissue were collected. The expression of D2-40 was detected by immunohistochemistry, and lymphatic microvessel density（LMVD）was determined by lymphatic endothelial specific marker D2-40. The correlations between LMVD and the clinicopathological characteristics were analyzed. Results： The mean value of LMVD in the peritumoral tissue was significantly higher than that in the intratumoral tissue（ 11.3 ± 4.6 vs 5.1 ± 2.1, P 〈 0.01 ）. The values of LMVD in rectal cancer intratumoral and peritumoral tissues were significantly higher than those in normal rectal tissue（3.8 ± 2.5, P 〈 0.01）. There was a positive significant correlation of high LMVD in peritumoral tissue with the infiltration （ P 〈 0.05）, lymph node metastasis, distant metastasis and Dukes stages （ P 〈 0.01）, but it had no significant correlation with the gender and age of patients, the dimension, location and differetion degree of tumors. Conclusion： High LMVD in peritumoral tissues is closely correlated with the lymph node metastasis in metal carcinoma. The detection of LMVD in peritumoral tissues may be the index which can be used to judge the lymphangiogenesis or lymph node metastasis of tumor.