苦石莲蛋白分离纯化及抗肿瘤作用探讨

Primary Study on Proteins Isolation and Purification from Caesalpinia Minax Hance and Its Anti-Tumor Effect

运用硫酸铵分级沉淀、阳离子交换和凝胶层析等方法,分离、纯化得到了一种苦石莲蛋白,其分子量约为29.64kD.采用MTT法考察了其抗肿瘤作用.结果表明,苦石莲蛋白对小鼠黑色素瘤细胞(B16)的体外增殖有明显抑制作用,呈现出明显的量-效关系和时-效关系.作用48 h,该蛋白对B16细胞的半抑制浓度(IC50)约为136μg/mL.

The Caesalpinia minax Hance protein （molecular weight being 29. 64 kD） is obtained by isolating and purifying steps of protein precipitation with ammonium sulfate, cationic exchanging chromatography and gel filtration. MTF is adopted to inspect its anti - tumor effect. It is shown through the results that the protein of Caesalpinia minax Hance has a perfect inhibition effect on the proliferation of mouse＇s melanoma cells （B16 cells）, which presents an .obvious quantity - effect relationship and time - effect relationship. Within 48 hours, the protein has an IC50 about 136 μg/mL on B16 cells.