杏香兔耳风组培苗根系发育解剖学研究

Anatomy on Roots Development for Tissue Cultural Plantlets of Ainsliaea fragrans

以杏香兔耳风组培生根苗同一个无性系的不同分株为试验材料,在生根培养过程中,采取每天取样的方法对组培苗不定根的发育进行系统的显微观测和分析。结果表明:杏香兔耳风组培苗根系产生的根原基源于髓射线分生细胞,根系发育过程可大致分为髓射线分生细胞分化、根原基产生和不定根形成3个阶段;完成根系发育所需的时间大约为13d。本研究可为杏香兔耳风组培生根培养条件的优化提供参考依据。

Taking different plants of the same clones of tissue culturerooted seedlings of Ainsliaea fragrans as test materials. With continuous collecting material a time for each day, the root development process in vitro was observed and analyzed systematically by micro-anatomizing in A insliaeafragrans in this paper. The results showed that the primordial for the adventitious root in vitro was originated in the pith ray ceils, the development process of the adventitious root could be divided into three stages, just as the pith ray meristematic cells differentiation stage, the primordial root formation stage and the adventitious root formation stage; it needed about 13 days for a cycle. This research can provide a foundation for optimizing the rooting culture conditions in vitro of A. fragrans.