补肾活血方对多囊卵巢大鼠卵巢MMP-2，9的表达干预

The Influences of Kindey - Tonifying Blood - Quicking Formula （KTBQF） on MMP - 2, - 9 Expression in Ovary of Rat PCO Model

目的观察补肾活血方对多囊卵巢（PCO）大鼠基质金属蛋白酶-2，9（MMP-2，9）表达的影响，探讨补肾活血方治疗PCO排卵障碍的机理。方法选用未成年24日龄SD雌性大鼠60只，随机分为模型对照组、西药对照组、补肾活血方高剂量组、补肾活血方低剂量组、正常对照组共5组，每组12只。建立大鼠PCO病理模型，采用原位杂交法检测各组卵巢组织中MMP-2，9基因的表达水平。结果经图象分析和统计处理，各组大鼠卵巢中卵泡的颗粒细胞和发育良好的卵泡膜细胞中均可检测到MMP-2和MMP-9的杂交信号。模型组MMP-2与MMP-9表达强度明显超过正常组、补肾活血方高剂量组、补肾活血方低剂量组及西药对照组（P〈0．05）；补肾活血方高剂量组MMP-2与MMP-9的表达接近正常组（P〉0．05）；补肾活血方低剂量组、，西药对照组与正常组相比，MMP-2与MMP-9的表达均较正常组高（P〈0．05）；补肾活血方低剂量组与西药对照组MMP-2与MMP-9的表达强度相似，但均超过补肾活血方高剂量组的表达强度（P〈0．01）。结论MMPs的过度表达可能是卵巢排卵障碍的关键因素，提示MMPs参与了卵巢排卵过程；大鼠PCO动物模型卵巢局部MMP-2，9异常高表达与PCO大鼠卵巢排卵障碍有关，补肾活血方通过抑制MMP-2，9的异常高表达，从而发挥治疗多囊卵巢排卵障碍的作用，这可能是其促进卵巢排卵的机制之-。

Objective To observe the influences of Kindey - Tonifying Blood - Quicking Formula （KTBQF） on MMP - 2, - 9 Expression in ovary of rat PCO model and to discuss the mechanism of KTBQF treating abnormal ovu- lating of PCO. Methods 60 SD female rats of 24 days age were chosen as research object, and divided into high dosage group of KTBQH, low dosage group of KTBQH, model group and western medicine group and normal control group at random. In contrast with Chloramiphene, the In situ hybridization was used to detect the MMP - 2, - 9 ex- pression in the rat ovary of the five groups. Results Through imaging and statistics analyzing, we could get the follow- ing results ： MMP - 2, - 9 were present in the granulosa and thecal cells of the ovary in the control group. MMP - 2 and MMP - 9 expression in granulosa and thecal cells of the ovary in the model group was much greater than that in the con- trol group, high dosage of KTBQF, low dosage of KTBQF and western medicine group （p 〈 0.05） . The expression of MMP - 2, - 9 in the granulosa and thecal cells of the ovary in the high dosage of KTBQF was as high as that in the con- trol group （p 〉 0.05） . Compared with the control group, the expression of MMP - 2, - 9 in the granulosa and thecal cells of the ovary in the low dosage of KTBQF and clomiphene MMP - 2, - 9 in the granulosa and thecal ceils of the ovary in group were much higher （p 〈 0.05） . The expression of the low dosage of KTBQF was as high as that in the western medicine group, but they were both higher than that in the high dosage of KTBQF （p 〈 0.01 ） . Conclusion All above showed that the animal model of rat PCO were successfully established. The over- expression was a key factor of abnormal ovulatinig in ovary, which showed MMPs might take part in the process of ovulating. Kindey - Tonifying Blood - Quicking Formula inhibited the over - expression of mmp - 2, 9 and could bring into a role of treating abnormal ovu- lating in PCO, which is one of the mechanism to promote ovulation.