造血干细胞移植后人巨细胞病毒动态监测的临床意义

Clinical Significance of Monitoring of Human Cytomegalic Inclusion Disease Virus after Haemopoietic Stem Cell Transplantation

【目的】探讨造血干细胞移植(HSCT)术后巨细胞病毒(CMV)感染的监测方法及意义。【方法】对40例进行HSCT的患者采用实时聚合酶链反应(PCR)技术定量动态监测白细胞中巨细胞病毒(CMV)DNA载量并进行比较分析。【结果】40例进行HSCT的患者中有12例出现阳性,阳性率为30.00%。1例死亡,病死率为2.50%。12例阳性患者均经CMV抗病毒治疗,其中1例死亡,11例转阴。阳性结果持续次数最长为21 d,最短为7 d。【结论】动态监测巨细胞病毒感染状况在移植群体中有着重要的价值。

[Objective] To discuss the ways of monitoring cytomegalovirus（CMV） infection after haemopoietic stem cell transplantation （HSCT） and its significance. [Methods]Real-time polymerase chain reaction technique was used to monitor DNA quantity of CMV in white blood cell and the comparison was made. [Results]Among 40 patients, there were 12 patients with high DNA quantity of cytomegalic inclusion disease virus beyond normal value, and the positive rate was 30.00%. One patient was dead, and the case fatality ratio was 2.50%. After anti-CMV therapy, there was 1 death, and CMV of another 11 patients was negative. The number of times of positive findings was 1 and 4, respectively, correspond to the longest and shortest. There was no marked change in results of 1 death. [Conclusion]The dynamic monitoring of CMV infection has important value for transplantation group.