反相高效液相色谱法测定医药中间体2-乙氧羰基环戊酮

Determination of Ethyl 2-oxocyclopentanecarboxylate by Reversed Phase High Performance Liquid Chromatograph

建立了一种用反相高效液相色谱法(RP-HPLC)测定医药中间体2-乙氧羰基环戊酮的方法。选用Kromasil C18(250mm×4.6mm,5μm)色谱柱,以甲醇-水(60∶40,V∶V)为流动相,流速为0.6mL/min,紫外检测波长为253nm,对药物中2-乙氧羰基环戊酮的含量进行测定。实验表明,2-乙氧羰基环戊酮在6～300μg/mL的浓度范围内,峰高与浓度呈良好的线性关系,线性方程为Y=13.873X-70.839,线性相关系数R2=0.9992,RSD=3.30%,回收率为98.02%～101.22%。最低检测限为0.08μg/mL。本法操作简单、灵敏度高,可准确快速地测定药物中2-乙氧羰基环戊酮的含量。

A method for the determination of ethyl 2-oxocylopentanecaroxylate by reversed phase high performance liquid chromatography is developed. Kromasil C18 （250 mm ×4.6 mm, 5 μm） is chosen as chromatographic column. Methanol-water （60： 40, V： V） at flow-rate of 0.6 mL/min is mobile phase with UV detection wave at 253 nm. The relationship between peak height and concentration in the range from 6 to 300 μg/mL show good linearity. RSD is 3.30%. The detection limit is 0.08μg/mL. The recovery of samples are 98.02% - 101.22%. It indicates that the proposed method is sensitive reliable and can be？ applied in analysis of medical materials.