吸毒人群中HIV及结核杆菌感染者CD4^+与CD8^+细胞检测分析

Monitoring Immunology Index CD4 and CD8 in Drug Users with HIV or Tuberculosis

目的对吸毒人群中HIV及结核杆菌感染者的CD4+、CD8+细胞进行监测,评价HIV和结核杆菌感染后的免疫学变化。方法采用流式细胞技术(FCM)对吸毒人群中9例仅HIV阳性者、5例仅患活动性结核病者、40例吸毒人群中健康者及40例正常人群,分别检测CD3+、CD3+CD4+、CD3+CD8+细胞百分率计算出CD3+CD4+/CD3+CD8+即Th细胞与Ts细胞的比值,分析各组免疫学检测结果的差异。结果HIV感染组、结核杆菌感染组及吸毒者健康组、正常人组各项指标间差异有统计学意义(F=50.24,F=63.13,F=92.51.F=107.29,P均<0.05),CD3+CD4+/CD3+CD8+细胞的比值均明显低于对照组,HIV<TB<吸毒人员健康者<正常人群(P<0.01)。结论CD3+CD4+/CD3+CD8+细胞比值的检测在评价HIV、结核杆菌感染者的免疫状况、进展和预后判断等方面具有重要作用。

Objective To estimate the immunologic variation, monitoring the immunology index CD4 and CD8 in drug users with HIV or tuberculosis. Methods Flow cytometer （FCM） was adopted to determine CD3^＋ ,CD3^＋ CD4^＋ , CD3^＋ CD8^＋ percentage, then calculate CD3^＋ CD4^＋/ CD3^＋ CD8^＋ ratio, between different groups of drug users and normal persons. Results There were differences of statistical significance in cell counts of CD3^＋ , CD3^＋ CD4^＋ , and CD3^＋ CD8^＋ among persons with 9 HIV, 5 TB, 40 drug users of good health, 40 normal persons, F test （ P 〈 0. 05, F = 46.05,63. 13,92.51,107.29）. CD3^＋ CD4^＋/ CD3^＋ CD8^＋ ratio （P 〈 0. 01 ） in persons with HIV was lower than in TB and in the control. Conclusion The detection of percentage of CD4 ceils and CD4/CD8 ratio may be important in evaluating immune condition, progression and prognosis of patients with HIV or TB infection. Moreover, it can be presumed that polyinfection with HIV and TB may hurt the patient＇ s immune function more seriously.