高效液相梯度洗脱法测定醋柳黄酮中的主要成分

Determination of the main component in TFH with Gradient elution by HPLC Method

目的:建立高效液相梯度洗脱法测定醋柳黄酮中的主要成分(槲皮素和异鼠李素),降低保留时间,提高检测效率。方法:Sinochrom-C18色谱柱(200 mm×4.6 mm,5μm);流动相:A:甲醇;B:磷酸水溶液,进样0 min^3 min后,调节流动相A为65%(体积比);3 min^9 min,流动相A为58%;9 min^30 min,流动相A为50%;流速:1.0ml/min;检测波长:372 nm;柱温:35℃,进样5μl。结果:槲皮素在1.6 mg/L^8.0 mg/L(r=0.9996),异鼠李素在2.0 mg/L^10.0 mg/L(r=0.9998)的浓度范围内线性关系良好,槲皮素和异鼠李素在醋柳黄酮中的含量分别为3.42%、11.07%。结论:该方法样品处理简单,测定结果准确度高,分离效果好,适用于该药的成分测定。

Objective： To determine the main component （ quercetin and isorhamnetin） in total flavonoes of Hippophae rhamnoides（TFH） with gradient elution by HPLC. Methods： The high performance liquid chromatography（HPLC） system consisted of Sinochrom-C18 column（200 mm × 4.6 mm, 5 μm） ; mobile phase of A： methanol, and B： phosphoric acid, after 3 minutes, the ratio of A was 65% ; between 3 and 9 minutes, the ratio of A was 58% ; between 9 and 20 minutes, the ratio of A was 50%. Flow rate was 1.0 mL. min i with detection 372 nm; column temperature at 35 ℃ ; sampling 5 μl. Results： The linear range of quercetin and isorhamnetin were 1.6-8.0 mg L^-1（r =0.9996） and 2.0 - 10.0 mg L^-1（r = 0. 9998）. The quantity of quercetin and isorhamnetin in TFH were 3.42% , 11.07%. Conclusion： This method is simple and accurate. It is suitable for the components and quantity determination.