摘要
采用柱前NDA衍生的方法，建立了毛细管电泳电化学检测牛磺酸的分析方法。考察了NDA衍生条件、检测电势、分离电压、缓冲溶液pH值、缓冲溶液浓度等实验参数对牛磺酸检测的影响。在最佳实验条件下，检测牛磺酸的浓度范围是5．00×10^-4-1．00×10^-3mol／L，线性回归系数为0．9997，浓度检出限LODe为1．60×10^-6mol／L（S／N=3）。以此方法检测了人血液样品中的牛磺酸的含量，方法的加标回收率在93％-95％之间。
A new method for the detection of taurine was developed using capillary electrophoresis by deriva - tiza- tion of 2,3 - naphthalene - dicarboxaldehyde （ NDA ） pre - column. The effects of the derivatization time, detec - ted potential, running buffer pH, running buffer concentration and separation voltage were studied. Under the opti- mum conditions of detection, the linear range of taurine was 5.00 ×10^-6 -1.00×10^-3mol/L. The corre - lation coefficient of the method was 0.9997. The concentration limit of detection LODc （ S/N =3 ） was 1.60×10^-6moL/ L. The method was used to detected taurine both in normal hemolysis and abnormal hemolysis. The recovery of the Jnethod was between 93% -95%.
出处
《青岛农业大学学报（自然科学版）》
2008年第4期311-314,318,共5页
Journal of Qingdao Agricultural University(Natural Science)
关键词
毛细管电泳
电化学检测
牛磺酸
衍生
capillary electrophoresis
electrochemical detection
taurine
derivatization
