红树林细菌Flavobacterium sp.的抑藻活性物质鉴定及其对亚历山大藻抑制作用的初步研究

Preliminary study on identification of algicidal active substance from mangrove bacteria Flavobacterium sp. and its algicidal effect on red tide algae Alexandrium tamarense

目的研究红树林细菌中抑制赤潮藻生长的活性物质。方法利用硅胶柱层析对广西红树林细菌Flavobacteriumsp.产的一种红色抑藻活性物质进行分离和纯化,通过波谱方法和参考文献数据鉴定其分子结构,并对其赤潮藻抑制作用进行了初步研究。结果与结论这种红色抑藻活性物质为灵菌红素。它可明显抑制塔玛亚历山大藻细胞的生长,抑制作用随灵菌红素的浓度加大而显著增强;可促使赤潮藻细胞MDA含量升高,使巯基含量下降,并使藻细胞膜透性增大;可以使赤潮藻细胞膜受到损伤,进而抑制藻细胞生长。

Objective To study on algicidal bioactive substances from mangrove bacteria and its algicidal effect on red tide algae Alexandriam tamarense. Methods A red algicidal bioactive substance in Guangxi mangrove bacteria was isolated and purified by Silica-gel Column Chromatography, and its structure was determined on the basis of UV, IR, ^1H NMR, ^13C NMR and HREIMS spectroscopic analyses and comparison with the literatures. Its algicidal affect was primarily studied in this paper. Results and Conelusion The results showed that the red algicidal bioactive substance was prodigiosin, which had an inhibitory effect on the cells growth of Alexandrium tamarense, and the inhibitory effect increased with the increase of prodigiosin concentration. The prodigiosin stimulated the production of MDA and reduced the content of sulfhydryl group. In addition, plasma membrane permeability of leaked cell solution was increased. These indicated the extinguishing mechanism, the cell membrane was damaged followed by the inhibition of the cell growth.