刺参糖胺聚糖对单纯疱疹病毒Ⅰ型抑制作用的实验研究

Study on the antiviral effects of Stichopus japonicus Selenka glycosaminoglycans (SJ-GAG) on HSV-Ⅰ

目的探讨刺参糖胺聚糖对单纯疱疹病毒（herpes simplex virus，HSV）Ⅰ型的抑制作用以及对感染小鼠的保护作用。方法以Vero细胞为靶细胞，接种HSV-1SM44毒株，同时分别加入不同浓度的刺参糖胺聚糖，观察细胞病变和定量PCR检测刺参糖胺聚糖的抗病毒作用；体内实验以HSV-1SM44毒株颅内接种感染小鼠，4h后以不同剂量的刺参糖胺聚糖灌胃，观察刺参糖胺聚糖对感染病毒小鼠的保护作用。结果刺参糖胺聚糖浓度在1．6mg·mL^-1以上时，表现出对Vero细胞的毒性作用；在0．25～0．2mg·mL^-1浓度范围表现出抗病毒活性，且具有一定的量效关系，对细胞无毒性作用。动物实验表明颅内感染小鼠刺参糖胺聚糖灌胃后，存活时间延长，死亡数明显减少；且呈一定正相关。结论刺参糖胺聚糖具有抗HSV-Ⅰ、保护HSV-Ⅰ感染小鼠的作用。

Objective To study the antiviral effects of SJ-GAG on herpes simplex virus Ⅰ （HSV- Ⅰ ） in vitro as well as its protective effect on infected mice. Methods The Vero cells were exposed to HSV- Ⅰ SM44 and different concentrations of SJ-GAG, respectively. Cytopathic effect（CPE） was observed and qunatitative PCR was used to evaluate the antiviral effect of SJ-GAG. In vivo experiment, the mice were infected with HSV1 intracranial vaccination and followed SJ-GAG intragastric administration 4h later to test the protection of SJ- GAG on HSV1 infected mice. Results When the concentration of SJ-GAG was above 1.6mg·mL^-1 ,it showed cytotoxieity. When the concentration was among 0.25-0.2mg·mL^-1 ,it expressed marked antiviral effect without cytotoxicity. SJ-GAG could prolong the survival duration of infected mice and decrease the mortality rate significantly. The protection of SJ- GAG showed a dose-effect relationship. Conclusion SJ-GAG has antiviral effect and shows some protective effect on HSV-1 infected mice.