不同基质体系中白腐菌对喹啉和吲哚的降解

Biodegradation of Quinoline and Indole by Pleurotus ostreatus in Different Substrate Systems

采用单基质及混合基质体系,探讨了白腐菌Pleurotus ostreatusBP对氮杂环化合物的降解规律.结果表明,在弱酸性环境下,白腐菌能有效降解喹啉和吲哚.单基质体系中,喹啉的降解率15d内能达到89.48%,98.17%的吲哚6d内被降解;混合基质体系中,基质(喹啉、吡啶、苯酚及氨氮)的加入能促进白腐菌对吲哚的降解,但(吲哚、吡啶、苯酚及氨氮)却抑制了白腐菌对喹啉的降解.不同基质体系中,白腐菌对喹啉和吲哚的降解分别遵循零级和一级反应动力学.相对单基质而言,混合基质的存在对白腐菌生物学特性产生明显影响,可缩短生长周期,提高生长速率和漆酶活性.漆酶在吲哚的降解中起着重要的作用,但不能完全决定喹啉的降解.

Biodegradation of quinoline and indole was studied with a white rot fungus Pleurotus ostreatus BP under different single substrates and mixing systems. The results showed that P. ostreatus could degrade quinoline and indole effectively under slightly acidic condition. With single substrate, the degradation rate of quinoline by P. ostreatus could reach 89.48% after incubation for 15 d and that of indole was about 99% for 6 d. In the mixing systems, the addition of mixing substrates, such as quinoline, pyridine, phenol and NH4^＋-N, could improve the degradation of indole by the fungus. However, the degradation of quinoline by the fungus was inhibited by the addition of mixing substrates, such as indole, pyridine, phenol and NH4^＋-N. Additionally, in the systems with different substrates, the biodegradation kinetics of quinoline and indole followed zero-order and first-order reaction kinetics, respectively. The biological characteristics of P. ostreatus were obviously affected by the mixed substrates, which could shorten the growth cycle, increase the growth rate of biomass and enhance the laccase activity. Furthermore, laccase played an important role in the degradation ofindole, did not in that of quinoline. Fig 5, Tab 2, Ref 14