四味甘温归肝肾经中药对性激素致大鼠肾阳虚的影响

Effects of Four Traditional Chinese Medicine with Sweet-flavor,Warm-nature,Meridian Tropism in Liver and Kidney on Kidney-yang Deficiency Rats Induced by Sex Hormone

目的观察杜仲、何首乌、海马、菟丝子四味归肝肾经中药对性激素致大鼠肾阳虚的影响,认识"甘,温,归肝、肾经"中药药性的现代科学内涵。方法采用腹腔注射苯甲酸雌二醇造成雄性大鼠肾阳虚模型、腹腔注射丙酸睾酮造成雌性大鼠肾阳虚模型。SD大鼠70只,随机分为7组:正常对照组、模型对照组、金匮肾气丸组、杜仲组、何首乌组、海马组、菟丝子组,每组10只,灌胃给药,每日1次,连续12 d。测定大鼠抓力、肛温、睾丸指数、附睾指数、前列腺指数、精囊腺指数、卵巢指数、子宫指数。结果①杜仲、海马、菟丝子三味中药均可提高肾阳虚大鼠抓力,因其均入肝肾经从而改善腰膝酸软。②杜仲、海马、菟丝子三味中药可升高肾阳虚大鼠的肛温,因其性温从而起温煦作用。③杜仲、何首乌可升高雄性肾阳虚大鼠的睾丸指数,海马可提高雄性肾阳虚大鼠精囊腺指数,菟丝子可恢复雄性肾阳虚大鼠前列腺指数,何首乌、菟丝子可改善雌性肾阳虚大鼠的卵巢指数,四味中药能不同程度改善肾阳虚证之性欲减退、肾精亏虚等症状与其入肾经有关。结论杜仲、海马、菟丝子、何首乌四味中药能不同程度改善肾阳虚证之腰膝酸软、畏寒肢冷、性欲减退、肾精亏虚等症状相关作用,这为探讨"甘温归肝肾经"中药的共同作用规律提供了新内容。

OBJECTIVE To observe the effects of four traditional Chinese medicine （ TCM ） such as Cortex Eucommiae, Radix Polygoni Multiflori, Hippocampus and Semen Cuscutae on kidney-yang deficiency rats induced by sex hormone, and understand the modern scientific connotation of the properties theories about sweet-flavor, warm-nature, in liver and kidney. METHODS Kidney- yang deficiency male rat ＇s model was established with intraperitoneal injection of estradiol benzoate, while kidney-yang deficiency female rat＇s model was established with intraperitoneal injection of testosterone propionate. A total of 70 healthy adult SD rats were divided into seven groups randomly： normal control group, model control group, positive control group （Jinkuishenqiwan）, four TCM （Cortex Eucommiae, Radix Polygoni Multiflori, Hippocampus and Semen Cuscutae） groups, 10 rats in each group and gastric infusion for 12 days, once a day. The effects were observed by measuring grip strength, rectal temperature, testis index, epididymis index, prostatic index, seminal vesicle index, uterus index and ovarian index. RESULTS ① Cortex Eucommiae, Hippocampus and Semen Cuscutae could markedly improve grip strength, which suggests that a relationship may exist between meridian tropism in liver and kidney and its effect on improving soreness of the waist and knees. ② Cortex Eucommiae, Hippocampus and Semen Cuscutae could markedly improve rectal temperature with the possible causes of its warm-nature. ③ Cortex Eucommiae and Radix Polygoni Multiflori could augment testis index of kidney-yang deficiency male rats. Hippocampus could increase seminal vesicle index of male rats. Semen Cuscutae could increase prostatic index of male rats. Radix Polygoni Multiflori and Semen Cuscutae could improve ovarian index of female rats. The possible cause for improving symptoms of hyposexuality and kidney-essence deficiency is its meridian tropism in kidney. CONCLUSION All the effects of the four TCM （ Cortex Eucommiae, Radix Polygoni Multiflori, Hippocampus and Semen Cuscutae） above could improve such symptoms of kidney-yang deficiency as soreness of the waist and knees, intolerance of cold and cold limbs, hyposexuality and kidney-essence deficiency in different degrees. It provides the experiment foundation for the common regularity of the properties theories about sweet-flavor, warm-nature, meridian tropism in liver and kidney.