抗瘤Ⅰ号胶囊抗肿瘤作用的体内实验研究

Experimental Study on Anti-tumor Activity of the Anti-lump Ⅰ Capsule

目的通过体内实验探讨抗瘤Ⅰ号胶囊的抗肿瘤作用。方法抗瘤Ⅰ号胶囊对小鼠宫颈癌(U14)、小鼠肝癌(H22)生长的抑制作用。采用常规无菌接种,接种后按体重随机分为6组。接种24 h后给药,末次给药24 h后颈椎脱臼法处死小鼠,称记体重,剥离瘤块并称重。计算各组动物试验前后体重及瘤重平均值,求其抑瘤率。瘤重抑制率=(对照组平均瘤重-给药组平均瘤重)/对照组平均瘤重×100%。抗瘤Ⅰ号胶囊对小鼠前胃癌FC肿瘤生长的抑制作用:剥离FC荷瘤小鼠皮下瘤块、称重,用生理盐水1∶7制成匀浆,于纯系615小鼠右腋皮下注射0.2 mL含1×105个癌细胞,其它方法同前。结果抗瘤Ⅰ号胶囊能明显抑制小鼠肿瘤U14,H22和FC的生长;与环磷酰胺合用对小鼠U14肿瘤的治疗有明显的增效作用;能明显提高环磷酰胺中毒小鼠的白细胞、淋巴细胞和骨髓有核细胞数量。结论抗瘤Ⅰ号胶囊具有一定的抗肿瘤效果。

OBJECTIVE To discuss the anti-tumor activity of anti-lump Ⅰ capsule through in vivo experiment. METHODS First,the inhibitory, action of the anti-lumpⅠ capsule on the mouse cervical carcinoma （U14） and the mouse hepatoma （H22） was studied. The mice were treated by the conventional aseptic vaccination and then divided into 6 groups according to the body weight. After 24 hours the mice were administered with anti-lump Ⅰ capsule. After 24 hours from the last administration, the mice were executed by cervical vertebra dislocation and weighed. They were weighed again after striping the tumor. The suppression rate was calculated though the mean values of the body and lump weight of each group animals： suppression rate = （ average lump weight of control group - average lump weight of treated group）/average lump weight of control group x 100%. Second, the inhibitory action of the anti-lump Ⅰ capsule on the mouse forestomaeh carcinoma （FC） was also studied. The hypodermic lump of FC Holland lump mouse was striped, weighed and homogenated with physiological saline. The pure line 615 mice＇ right armpits were treated by hypodermic injection with 0.2 mL homogenate containing 1 × 10^5 cancer cells. Other methods were carried out as the above mention. RESULTS The anti-lump Ⅰ capsule can obviously suppress the growth of mouse tumor U14, H22 and FC. The synergistic effect on the mouse U14 tumor with the endoxan was existed. The anti-lump Ⅰ capsule could obviously enhance the number of white blood cell, the lymphocyte and the marrow nucleated cell of poisoned mice educed by endoxan. CONCLUSION The anti-lump Ⅰ capsule has the certain anti-tumor activity