亚麻籽提取物中开环异落叶松树脂酚二葡萄糖苷含量的测定

Determination of Secoisolariciresinol Diglucoside in Flax Seed Extract by HPLC

目的建立亚麻籽提取物中开环异落叶松树脂酚二葡萄糖苷（SDG）含量的HPLC测定分析法。方法色谱条件为Hypersil ODS C18（4．6mm×250mm，5μm），流速1．5mL·min^-1，柱温25℃，检测波长220nm，用乙腈-0．12％磷酸溶液作为流动相进行梯度洗脱。结果SDG在0．3～6．0μg内与峰面积呈良好的线性关系，r=0．9999。平均加样回收率为99．7％，RSD为1．13％。结论本方法精确可靠，可作为亚麻籽提取物质量控制的方法。

OBJECTIVE To establish a rapid and sensitive high-performance liquid chromatographic assay for the determination of secoisolariciresinol diglucoside （SDG） in Flax seed extract. METHODS Chromatography was performed on a Hypersil ODS C18 （4.6 rum ×250 mm,5 μm） at 220 nm. The mobile phase was gradient elution with acetonitrile-0.12% phosphoric acid solution at a flow rate of 1.5 mL · min^ -1. RESULTS The linear range of SDG was 0.3 - 6.0 μg with a correlation coefficient of 0. 999 9. The average recovery was 99.7% and RSD 1. 13%. CONCLUSION The method is accurate and reliable for the quality control of Flax seed extract.