摘要
目的建立同时测定心可舒片中3种有效成分——葛根素、丹参酮ⅡA和熊果酸含量的HPLC。方法C18色谱柱,梯度洗脱,流动相为乙腈.水(含0.3%磷酸),流速为1.0mL·min^-1,柱温为25℃,MWD检测器。结果葛根素、丹参酮ⅡA和熊果酸的线性范围分别为52.6—262.8,4.2~21.1和20.5~102.6μg·mL^-1,r值分别为0.9997,0.9999和0.9997;平均加样回收率分别为100.63%,97.08%和96.92%,RSD分别为1.4%,1.3%和1.4%。结论该方法准确,操作简便,又可降低质量检验成本,可以用于心可舒片的质量控制。
OBJECTIVE To establish an HPLC method for simultaneous determination of puerarin, tanshinone Ⅱ A and ursolic acid in Xinkeshu tablets as three major bioactive components. METHODS A C18 column was used. The chromatography was carried out with a stepwise gradient programming. The mobile phase was acetonitrile-water( containing 0.3% phosphorous) and the flow rate was 1.0 mL · min^-1. RESULTS The linear ranges of puerarin, tanshinone Ⅱ A and ursolie acid were 52.6 - 262.8 μg · mL^-1, r = 0. 999 7, 4.2 - 21.1 μg · mL^-1 , r = 0. 999 9 and 20.5 - 102.6μg · mL^-1, r = 0. 999 7, respectively. The average recovery rates of puerarin, tanshinone Ⅱ A and ursolic acid were 100.63% with RSD 1.4% , 97.08% with RSD 1.3% and 96.92% with RSD 1.4% , respectively. CONCLUSION The method is successfully applied to analyze three major bioactive components in Xinkeshu tablets. This method is simple, accurate and effective for the quality control of Xinkeshu tablets.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2008年第6期536-539,共4页
Chinese Journal of Modern Applied Pharmacy