HPLC同时测定心可舒片中3种有效成分

The Simultaneous Determination of Three Major Bioactive Components in Xinkeshu Tablets by HPLC

目的建立同时测定心可舒片中3种有效成分——葛根素、丹参酮ⅡA和熊果酸含量的HPLC。方法C18色谱柱，梯度洗脱，流动相为乙腈．水（含0．3％磷酸），流速为1．0mL·min^-1，柱温为25℃，MWD检测器。结果葛根素、丹参酮ⅡA和熊果酸的线性范围分别为52．6—262．8，4．2～21．1和20．5～102．6μg·mL^-1，r值分别为0．9997，0．9999和0．9997；平均加样回收率分别为100．63％，97．08％和96．92％，RSD分别为1．4％，1．3％和1．4％。结论该方法准确，操作简便，又可降低质量检验成本，可以用于心可舒片的质量控制。

OBJECTIVE To establish an HPLC method for simultaneous determination of puerarin, tanshinone Ⅱ A and ursolic acid in Xinkeshu tablets as three major bioactive components. METHODS A C18 column was used. The chromatography was carried out with a stepwise gradient programming. The mobile phase was acetonitrile-water（ containing 0.3% phosphorous） and the flow rate was 1.0 mL · min^-1. RESULTS The linear ranges of puerarin, tanshinone Ⅱ A and ursolie acid were 52.6 - 262.8 μg · mL^-1, r = 0. 999 7, 4.2 - 21.1 μg · mL^-1 , r = 0. 999 9 and 20.5 - 102.6μg · mL^-1, r = 0. 999 7, respectively. The average recovery rates of puerarin, tanshinone Ⅱ A and ursolic acid were 100.63% with RSD 1.4% , 97.08% with RSD 1.3% and 96.92% with RSD 1.4% , respectively. CONCLUSION The method is successfully applied to analyze three major bioactive components in Xinkeshu tablets. This method is simple, accurate and effective for the quality control of Xinkeshu tablets.