摘要
目的:建立厚朴制剂藿香正气颗粒中厚朴酚含量测定的HPLC法。方法:采用甲醇溶解样品超声制备供试液;HPLC色谱条件为:Zorbax RP-C18色诸柱(150mm×4.6mm,5μm);流动相:甲醇-水(80:20,v/v);流速:1.0mL/min;检测波长:294 nm。结果:厚朴酚的线性范围是0.02至4μg/mL,其线性相关系数R=0.9992,藿香正气颗粒中厚朴酚的含量为0.273mg/袋,其厚朴酚加样回收率为99.2%至101.4%,RSD为1.73%。结论:此方法快速简便,可用于评价藿香正气颗粒的质量。
Objective：Determination of magnolol in Huoxiangzhengqi granules was established by HPLC.Method：Dissolved in methanol,sample solution of Huoxiangzhengqi granules was manufactured by means of ultrasoni.High performance liquid chromatography（HPLC） has the condition as below：column： Zorbax RP-C18 Chromatography column（150×4.6 mm,5 μm）;mobile phase： methanol： water（80：20,v/v）;flow rate： 0.5mL/min;λ=294nm Result： A good linear response towards magnolol was attained from 0.02μg/mL to 4μg/mL,（r=0.9992）.The content of ntagnolol in Huoxiangzhongqi granule was 0.273 mg/granule. Conclusion： The method is simple, connvenient and rapid, which will be helpful to quality assessment of Huoxiangzhengqi granule.
出处
《激光杂志》
CAS
CSCD
北大核心
2008年第5期96-96,共1页
Laser Journal
