海藻酸钠寡糖对烟草幼苗生长及光合特性的影响

Effects of Alginate Oligosaccharides on Growth and Photosynthetic Characters of Tobacco Seedlings

采用酶解法获得海藻酸钠寡糖,应用不同浓度的海藻酸钠寡糖处理烟草幼苗,结果表明:0.5mg.g-1海藻酸钠寡糖对烟草幼苗生长有促进作用,幼苗株高、叶面积、根长等生长指标与对照相比均显著增加;功能叶片的叶绿素含量比对照增加48.27%,净光合速率(Pn)为13.9μmol.m-2.s-1,气孔导度(Gs)为249m mol.m-2.s-1,蒸腾速率(Tr)为4.3m mol.m-2.s-1,胞间CO2浓度(Ci)为268μL.L-1,各项指标均显著升高;气孔限制值(Ls)为21%,显著低于对照(47%),并且施用海藻酸钠寡糖2次的效果优于1次。

Alginate oligosaccharides were prepared through enzyme decomposition. Different concentrations of alginate oligosaccha- rides were used in tobacco seedlings. The results indicated that 0.5mg,g^-1 of alginate oligosaccharides promoted the growth of to- bacco seedlings. The height of seedlings and the areas of functional leaves increased significantly （p〈0.05） compared with Ck. The content of chlorophyll was 48.27% higher than that of CK. The net photosynthetic rate （Pn） wasl3.9μmol.m-2.s-1. The stom- atal conductance （Gs） was 249mmol.m-2.s-1. The intercellular CO2 concentration （Ci） was 268μL.L-1 and the transpiration rate （Tr） was 4.3mmol.m-2.s-1. The stomatal limitation （Ls） was 21%, significantly （p〈0.05） low compared with that of CK（47%）. The effect of twice alginate oligosaccharides application was better than that of once application.