光聚合制备琥珀酸氯霉素分子印迹聚合微粒及分子识别特性研究(英文)

Studies on the Synthesis of Molecularly Imprinted Polymers of Chloramphenicol Succinate by Photopolymerization and Its Properties of Specifically Molecular Recognition

为制备对琥珀酸氯霉素(HS-CAP)分子具有特异性识别能力的分子印迹聚合微粒,并进一步应用于电化学传感检测琥珀酸氯霉素残留。实验采用快速紫外光引发聚合的方法,以HS-CAP分子为模板,以甲基丙烯酸(MA)为功能单体,以乙二醇二甲基丙烯酯(EGDMA)为交联剂制备了HS-CAP分子印迹聚合微粒。通过电镜扫描分析了该聚合微粒的表征,同时通过紫外分光光度法和Scatchard方程分析,研究了印迹聚合微粒的吸附性能及动力学特征。结果表明该方法制备的印迹聚合微粒表面存在大量可以特异性识别模板分子印迹微孔,直径介于0.2～0.5μm之间,印迹聚合微粒最大吸附量可达到13.66μmoL·g-1,平衡离解常数为3.75mmoL·L-1,印迹聚合微粒对HS-CAP分子的选择性吸附作用在40min内基本达到平衡。本实验制备HS-CAP分子印迹聚合微粒操作简单,印迹微粒对模板分子特异性识别能力强,为进一步制备HS-CAP分子印迹聚合膜提供了参考和依据。

To prepare the molecularly imprinted micro-particles with capacity of specifically recognizing chloramphenieol suceinate（HS-CAP） molecules and for its further use in the determination of HS-CAP residue by vultammetric sensor, in this study, photo-polymerization method was applied for the synthesis of the molecularly imprinted micro-particles of HS-CAP using methylacrylie acid as functional monomer, Ethylene glycol dimethaerylate as cross-linking agent and HS-CAP as template. Superficial characteristic of the imprinted micro-particles was analyzed by electron microscope scanning. Ultraviolet speetrophotometry and Seatehard equations were used for the investigation of the adsorption ability and its kinetics. A great number of imprinted micro-pores with specific recog-nizing sites was formed in the surface of the imprinted miero-particles with a diameter ranging over 0.2-0.5μm. Maximum adsorption quantity of the imprinted micro-particles reaches as high as 13.66μmoL·g^-1 and equilibrium dissociation constant is 3.75mmoL·L^-1. The preparation of moleeulaly imprinted micro-particles for HS-CAP in this study is simple and the resultant imprinted raiero-particles have strong recognizing eapability towards template molecules, and simultaneously this study has provided references and basic theory for the further studies of HS-CAP imprinted membrane.