培养条件对大肠杆菌产L-天冬酰胺酶的影响

Influences of Incubating Conditions on E.coli Accumulating L-asparaginase

研究了培养温度、培养时间、初始pH值和摇床转速等培养条件对所选大肠杆菌(E.coli№TN1.5885)产L-天冬酰胺酶的影响。实验结果表明,该菌株进行摇瓶培养的最适培养条件为37℃下培养16 h,初始pH值在7.0～7.5间,摇床转速约为300 r/min。其比酶活力约为406 U/g,比优化前大约提高了20%。

The influences of incubating conditions, including incubating temperature, incubating time, initial pH value and rotating speed of shaking table on E.coli （No TN1.5885） accumulating L-asparaginase were studied. The results show that the specific activity of L-asparaginase reached 406 U/g, 20% higher than that before optimization under the optimum conditions of incubating temperature 37℃, incubating time 16 h, the initial pH value between 7.0-7.5 and the rotating speed of shaking table 300 r/min.