导入玉米花色素苷调节基因Lc改变烟草花色——基因工程实验教学拓展

Changing flower color by introducing the anthocyanin regulatory gene Lc from Maize into tobacco——an experiment for gene engineering teaching

组织部分学生,将含有玉米花色素苷调节基因Lc的烟草叶片再生获得转基因植株,采用PCR,PCR-Southern blot和RT-PCR对转基因烟草进行检测,并观察性状变化.转基因烟草植株花冠筒、花冠檐、花丝颜色都与对照有明显差异.将直观性明显的转基因研究作为基因工程实验教学内容的拓展,不仅能够使学生更容易理解和掌握基因工程的特点,还有可能提高学生进行基因工程实验研究的兴趣.

A gene engineering experiment finished by some students and teacher togethers would be introduced in this paper. The transgenic tobacco plants were obtained by regenerating leaf blades with the anthocyanin regulatory gene Lc from maize. Polymerase chain reaction and PCR - Southern blot analyses demonstrated that transgenic tobacco plants contained transfer DNA （ T - DNA） with the Lc gene ; The result of RT - PCR analysis indicated Lc gene could express in transgenic tobacco plants ; There were distinct differences in the color of the flower tube, limb and filament between the transgenic lines and untransformed control. That transgenic researches with distinctly visual changes in phenotype were introduced into the experimental teaching of gene engineering could help students easily understand and master the characteristics of gene engineering, and improve their interest in doing experiments.