瘢痕疙瘩和正常皮肤成纤维细胞对白介素-1β和白介素-6的反应

The responses of fibroblasts from three parts of keloids and normal skin to interleukin-1β and interleukin-6

目的研究瘢痕疙瘩浸润、增生、老化3个不同部位的成纤维细胞性状。方法以6例瘢痕疙瘩为标本,6例正常皮肤为对照,通过细胞培养的疗法研究了瘢痕疙瘩3个不同部位和正常皮肤的成纤维细胞对白介素-1β(IL-1β)(200U/ml)和白介素-6(IL-6)(100U/ml)的反应。结果 IL-1β抑制瘢痕疙瘩增生部成纤维细胞而剌激正常皮肤的成纤维细胞,对浸润及老化部成纤维细胞无明显影响。IL-6抑制所有的成纤维细胞。结论瘢痕疙瘩不同部位和正常皮肤的成纤维细胞对 IL-1β和 IL-6的反应不尽相同。

Objective The purpose of this study was to explore the responses of fibroblasts from keloids and normal skin to interleukin-1β and interleukin-6.Methods Six samples of keloids and 6 sam- ples of normal skin were collected as the experimental and control group respectively.The means of cell culture was used to investigate the responses of fibroblasts from three different parts of keloids and nor- mal skin to interleukin-1β (200U/ml) and interleukin-6(100U/ml).Results Interleukin-1β could inhib- it the growth of fibroblasts from the proliferative part of keloids but stimulate growth of those from normal skin,while it did not affect the growth of those from other parts of keloids.Fibroblasts from dif- ferent parts of keloids and normal skin were all inhibited by interleukin-6.Conclusion The responses of fibroblasts from three parts of keloids and normal skin to interleukin-1β and interleukin-6 were not much similar.