摘要
目的观察不同复合皮移植后皮片的生长和收缩情况。方法对异体乳猪真皮+自体表皮(组1),异体小猪其皮+自体表皮(组2)和自体表皮(组3)共3个组移植的皮片,结合皮片完整性和猪妊娠过程等因素,经2年多动态监测移随皮片面积。结果移植后2～124周复合皮表面光滑无瘢痕;36周后3组皮片面积均有不同程度生长扩大,依次为组1>组2>组3(P<0.01);在复合皮组,24周后整张皮片明显优于拼接皮(P<0.001);猪妊娠过程显示,复合皮组有较好的组织增生能力和弹性回缩力(P<0.01);组织学观察组1乳头和皮钉等组织结构成熟早,24周时已接近正常皮肤。结论经酶处理的冷冻异体乳猪真皮复合移植后具有良好的组织弹性、耐磨性和细胞生长能力,是理想的真皮替代物;实验动物妊娠可客观地用于复合皮质量的检测。
Objective To evaluate the growth and contraction of different composite skin grafts.Methods The status of the following composite skin grafts was evaluated over 9 years :allogene- ic sucking pig dermis and autologous epidermis(group 1,n=19),allogeneic young pig dermis and autolo- gous epidermis (group 2,n= 7),autologous epidermis (group 3,n=8).The effects of gestation on the composite skin grafts were also assessed.Results It showed that composite skin grafts had smooth sur- face and no scar formation during 2 to 124 weeks after transplantation.After week 36,the surface area of each group enlarged with different growth rates :group 1>group 2>group 3(P<0.01).Whole piece grafting was significantly better than joining together of smaller pieces after week 24(P<0.001).Dur- ing pregnancy,CS had good growth ability and elasticity (P<0.01).Histological study showed that group 1 had rapid growth of skin papillae and epidermal ridges similar to normal skin at week 24.Con- clusion As CS graft with trypsin treated allogeneic sucking pig dermis as a substitute for dermis showed the advantages of rapid growth,good elasticity and stability,it is suggested that it could be used in clinical practice.
基金
国家自然科学基金资助
