Effects of indometacin on joint damage in rat and rabbit

目的：研究吲哚美辛（Ｉｎｄ）对关节损伤的影响．方法：检测佐剂性关节炎大鼠（ＡＡ）非致炎侧后足爪容积，ＬＰＳ诱导腹腔巨噬细胞和关节滑膜细胞产生白细胞介素１（ＩＬ１），以及兔滑膜成纤维细胞增殖反应和软骨蛋白多糖的合成．结果：Ｉｎｄ２ｍｇ·ｋｇ－１·ｄ－１ｉｇ９ｄ，可抑制ＡＡ大鼠ｄ１８，ｄ２１，和ｄ２４继发炎症反应，但促进巨噬细胞和滑膜细胞分泌ＩＬ１．Ｉｎｄ１０μｍｏｌ·Ｌ－１体外分别促进ＩＬ１诱导兔滑膜成纤维细胞增殖反应以及抑制关节软骨蛋白多糖合成．结论：Ｉｎｄ不利于关节损伤的修复．

AIM: To study the effects of indometacin (Ind) on joint damages. METHODS: The volume of noninjected hind paw and interleukin 1 (IL 1) production from peritoneal macrophages and articular synoviocytes induced by lipopolysaccharides were assayed in adjuvant arthritis (AA) rats. Measurements of synovial fibroblast proliferative response and proteoglycan synthesis of cartilage from rabbits were used. RESULTS: The secondary inflammatory reactions in AA rats on d 18, 21, and 24 were suppressed by ig Ind 2 mg·kg -1 ·d -1 for 9 d. Ind promoted IL 1 production from both macrophages and synoviocytes in AA rats. Ind 10 μmol·L -1 enhanced the proliferation of rabbit synovial fibroblasts and suppressed the proteoglycan synthesis of articular cartilage in response to IL 1 in vitro . CONCLUSION: Ind is unfavorable to the repair of joint destruction.