Fresh vs aged benzylpenicilin on non-IgE responses in mice

Fresh vs aged benzylpenicillin on non IgE responses in mice

目的：研究新鲜青霉素和陈旧青霉素是否能诱导不同的非ＩｇＥ抗体应答．方法：酶联免疫吸附试验测定抗体应答，半抗原抑制试验检测被抗体识别的抗原分子和抗原性交叉反应．结果：新鲜青霉素免疫后诱导的特异性非ＩｇＥ抗体类型主要是ＩｇＭ，其次是ＩｇＧ和ＩｇＡ．部分抗体识别青霉素分子本身，而大部分抗体结合降解产物或转化产物．青霉素和氨苄西林间交叉反应性抗体主要是ＩｇＧ，青霉素和哌拉西林间交叉反应性抗体则主要是ＩｇＭ．结论：新鲜制备的青霉素与陈旧青霉素能诱导不同的非ＩｇＥ抗体应答．

AIM: To study whether or not the freshly prepared benzylpenicillin could induce different non IgE antibody response from aged benzylpenicillin. METHODS: Antibody response was determined by enzyme linked immunosorbent assay (ELISA). Antigen molecules recognized by antibodies and antigenic cross reactions were tested by hapten inhibition assay. RESULTS: Isotypes of specific non IgE antibodies induced by freshly prepared benzylpenicillin were mainly IgM, and then IgG and IgA. Some parts of specific antibodies recognized benzylpenicillin molecule and major parts combined with degraded or transforming products. Isotypes of antibodies responsible for cross reaction were mainly IgG between benzylpenicillin and ampicillin and IgM between benzylpenicillin and piperacillin. CONCLUSION: Freshly prepared and aged benzylpenicillin induced different non IgE antibody response.