Effect of catecholamic acid on detoxication and distribution of NiCl_2 in mice and rats

目的：研究双酚胺酸（ＣＢＭＩＤＡ）对氯化镍的解毒作用．方法：ＮｉＣｌ２中毒后，立即给予ＣＢＭＩＤＡ，记录动物存活数；小鼠ｉｖ６３ＮｉＣｌ２后给药，测定２４ｈ组织中６３镍；用整体放射自显影术，显示小鼠体内６３镍分布．结果：ｓｃＣＢＭＩＤＡ０５－１５ｇ·ｋｇ－１对ｉｐＮｉＣｌ２５００ｍｇ·ｋｇ－１有解毒作用；小鼠ｉｐＮｉＣｌ２ＬＤ５０为８２８ｍｇ·ｋｇ－１，给药１５或２５ｇ·ｋｇ－１，ＬＤ５０分别为７８９和８２０ｍｇ·ｋｇ－１；大鼠ｉｍＣＢＭＩＤＡ５００ｍｇ·ｋｇ－１使ＮｉＣｌ２的ＬＤ５０提高８倍；组织中６３镍测定和定位显示，ＣＢＭＩＤＡ减少肺和血液中６３镍，增加了骨中６３镍，２４ｈ尿、粪６３镍排出与对照组无明显差异．结论：ＣＢＭＩＤＡ有效地解除镍毒性，提高动物存活率，降低镍在肺部的滞留．

AIM: To study the effect of catecholamic acid (CBMIDA) on detoxication of NiCl 2. METHODS: Mice and rats were injected sc or im CBMIDA immediately after ip NiCl 2. Each mouse was injected ip CBMIDA after iv 63 NiCl 2 185 kBq, and radioactivities of various tissues were measured with liquid scintillation counter at 24 h. The localization of 63 Ni was shown by the whole body autoradiography. RESULTS: CBMIDA sc 0 5-1 5 g·kg -1 markedly reduced the mortality from acute poisoning of ip NiCl 2 500 mg·kg -1 . After ip NiCl 2 in mice, the LD 50 was 82 7 mg·kg -1 . Mice were injected sc CBMIDA 1 5 or 2 5 g·kg -1 after Ni poisoning, the LD 50 of NiCl 2 were raised to 789 or 820 mg·kg -1 , respectively. The LD 50 of NiCl 2 was 39 mg·kg -1 in rat. If CBMIDA was injected im 0 5 g·kg -1 after ip NiCl 2, the LD 50 was 332 mg·kg -1 . CBMIDA 1 5 g·kg -1 im after iv 63 Ni Cl 2, decreased the contents of 63 Ni in blood and lung of mice vs control mice at 24 h. The contents of 63 Ni in brain, heart, spleen, and kidney were similar to those of the control mice. The content of 63 Ni in bone was more than the control. The excretions of 63 Ni through urine and feces were not increased by CBMIDA at 24 h. The whole body autoradiograpy showed that the radioactivity was highly localized in the kidney, lung, and Harders gland. There was a moderate level of 63 Ni in the liver, bone, skin, and blood. A pronounced accumulation occurred in the bone. There was a marked reduction of 63 Ni in the lung, skin, liver, and blood after ip CBMIDA. CONCLUSION: The CBMIDA markedly raised the survival rate of nickel poisoned mice and rats, and decreased 63 Ni levels in lung and blood.