庆大霉素毫微球的体内抗菌活性研究

In vivo antimicrobial activity of gentamicin polybutylcyanoacrylate nanoparticles

目的：观察庆大霉素与聚氰基丙烯酸正丁酯毫微球结合以后，其体内抗菌活性（特别是对细胞内感染）是否有所增加。方法：以鼠伤寒沙门杆菌感染的Ｃ５７ＢＬ／６ｊ小鼠为细胞内感染的动物模型，以小鼠存活率和器官组织中的活菌计数为指标，对庆大霉素聚氰基丙烯酸正丁酯毫微球的体内抗菌活性进行评价。结果：从存活率评价其治疗指数提高了１０倍。肝、脾、肾中活菌计数最低可降至庆大霉素溶液组的１／４２６，１／１４１和１／３０。结论：与庆大霉素溶液相比，庆大霉素毫微球明显提高了对伤寒沙门杆菌感染小鼠的治疗效果。

OBJECTIVE: To observe whether the in vivo antimicrobial activity of gentamicin (specially in the intracellular infected animals) could be improved after associting with polybutylcyanoacrylate nanoparticles. METHOD: S. typhimurium infected C57BL/6j mice were chosen as the animal model of the intracellular infection, then the antimicrobial activity of gentamicin polycyanoeacrylate nanoparticles (GMNP) was evaluated by the mouse survival and the bacterial counts. RESULTS: By comparison with gentamicin solution (GMSol), the therapeutic index of GMNP based on mouse mortality was incrased by 10 fold, and the bacterial counts on liver, spleen and kidney could be as low as 1/426, 1/141 and 1/30 of GMSol treated groups respectively. CONCLUSION: By comparison with GMSol, in vivo antimicrobial activity of gentamicin over S. typhimurium infected C57BL/6j mice improved significantly after associting with polybutylcyanoacrylate nanoparticles.