摘要
目的比较分析rIL-2刺激对人脐带血和成人外周血淋巴细胞亚群及功能的影响。方法利用流式细胞技术对脐带血和成人外周血淋巴细胞亚群进行检测,用t检验做统计学分析。结果新鲜未刺激的脐带血淋巴细胞在发育和功能方面均比成人外周血幼稚;两者对rIL-2刺激的反应明显不同,脐带血的变化主要表现为CD4+和CD8+细胞亚群的成熟和记忆功能增强,即由CD45RA+向CD45RO+细胞转化,而成人外周血淋巴细胞主要为CD33-CD16+的NK细胞比例增加和CD4+和CD8+细胞亚群的CD25+细胞比例增加,而CD45RA+和CD45RO+细胞亚群无明显变化。
The flow cytometry was used to compare and analyse the effect of stimulation of rIL2 on the lymphoid subpopulations and its functions in human umbilical cord blood (UCB) and adult peripheral blood (APB).The results showed that the freshly isolated lymphocytes from UCB were immature in terms of phenotypes and in function compared with those from APB.The responsiveness to rIL2 was also significantly different between them.The changes of UCB lymphocytes were mainly in the CD4+ and CD8+ subsets,that was increase in the degrees of their maturation and the memory function or the subsets shifting from CD45RA+ to CD45RO+.The changes in APB lymphocytes were mainly increases of percentages of CD33-CD16+ NK cells in lymphocytes’ population and of CD25+ cells in CD4+ and CD8+ subsets.The changes of CD45RA+ and CD45RO+ were insignificant.The compositions and responsiveness to rIL2 of lymphocytes of both UCB and APB were different.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1998年第1期12-15,共4页
Chinese Journal of Microbiology and Immunology
基金
广东省自然科学基金
关键词
脐带血
白细胞介素2
淋巴细胞亚群
流式细胞仪
Umbilical cord blood\ \ Interleukin2
Lymphocyte
Subsets\ \ Flow cytometry