摘要
目的观察CoxB3感染大鼠单个核细胞,诱导细胞表面LFA-1表达的变化,以及LFA-1/ICAM-1在CoxB3从单个核细胞向心肌细胞播散感染中的作用。方法制备和培养大鼠心肌细胞和大鼠外周血单个核细胞,并进行病毒感染;制备和纯化分泌抗大鼠LFA-1(CD11a)和抗大鼠ICAM-1(CD54)的单克隆抗体;在Wish细胞上进行病毒感染性滴定,并观察细胞病变;流式细胞仪分析各组单个核细胞LFA-1的表达情况;观察抗-LFA-1和抗-ICAM-1单克隆抗体在CoxB3复制和播散感染中的作用。结果CoxB3感染大鼠外周血单个核细胞18~24小时可以诱导细胞膜LFA-1表达增多,同时释放感染性病毒颗粒感染正常大鼠心肌细胞;加入抗-LFA-1或抗-ICAM-1的单克隆抗体均可不同程度地抑制病毒向心肌细胞播散感染,并显著降低培养上清液中病毒滴度。
Although Coxsackie B virus(Cox B) are known to infect various cells,virus-induced modulation of adhesion molecules on infected cells or adjacent cells,which possibly is a key factor to forster the dissemination of this virus,has been poorly understood.In this study,we examinated the expression changes of leukocyte function antigen 1(LFA1) on rat mononuclear cells infected with Coxsackievirus B3(Cox B3),and observed the roles of LFA1/ICAM1 on Cox B3 dissemination from mononuclear cells to cardiac myocytes.Cox B3 infection of rat peripheral blood mononuclear cell cultures for 18-24 hours was shown to induce changes in the expression of LFA1,and release infectious virus to infect normal rat myocytes.Addition of monoclonal antibodies to LFA1 or ICAM1 were found to block virus dissemination from infected mononuclear cells to normal myocytes,and to dicrease titers of virus in cultures.These data suggest that Cox B3 modulation of LFA1 expression on leukocytes might be an important step in virus pathogenesis.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1998年第1期24-28,共5页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金
关键词
柯萨奇B3病毒
白细胞功能抗原
细胞间粘附分子
Coxsackievirus B3\ \ Leukocyte function antigen 1\ \ Intercellular adhesion molecular1\ \ Antibody,monoclonal