LFA-1/ICAM-1在柯萨奇B组病毒播散感染中的作用初探

Effects of LFA1/ICAM1 on Coxsackievirus B3 dissemination

目的观察ＣｏｘＢ３感染大鼠单个核细胞，诱导细胞表面ＬＦＡ－１表达的变化，以及ＬＦＡ－１／ＩＣＡＭ－１在ＣｏｘＢ３从单个核细胞向心肌细胞播散感染中的作用。方法制备和培养大鼠心肌细胞和大鼠外周血单个核细胞，并进行病毒感染；制备和纯化分泌抗大鼠ＬＦＡ－１（ＣＤ１１ａ）和抗大鼠ＩＣＡＭ－１（ＣＤ５４）的单克隆抗体；在Ｗｉｓｈ细胞上进行病毒感染性滴定，并观察细胞病变；流式细胞仪分析各组单个核细胞ＬＦＡ－１的表达情况；观察抗－ＬＦＡ－１和抗－ＩＣＡＭ－１单克隆抗体在ＣｏｘＢ３复制和播散感染中的作用。结果ＣｏｘＢ３感染大鼠外周血单个核细胞１８～２４小时可以诱导细胞膜ＬＦＡ－１表达增多，同时释放感染性病毒颗粒感染正常大鼠心肌细胞；加入抗－ＬＦＡ－１或抗－ＩＣＡＭ－１的单克隆抗体均可不同程度地抑制病毒向心肌细胞播散感染，并显著降低培养上清液中病毒滴度。

Although Coxsackie B virus(Cox B) are known to infect various cells,virus-induced modulation of adhesion molecules on infected cells or adjacent cells,which possibly is a key factor to forster the dissemination of this virus,has been poorly understood.In this study,we examinated the expression changes of leukocyte function antigen 1(LFA1) on rat mononuclear cells infected with Coxsackievirus B3(Cox B3),and observed the roles of LFA1/ICAM1 on Cox B3 dissemination from mononuclear cells to cardiac myocytes.Cox B3 infection of rat peripheral blood mononuclear cell cultures for 18-24 hours was shown to induce changes in the expression of LFA1,and release infectious virus to infect normal rat myocytes.Addition of monoclonal antibodies to LFA1 or ICAM1 were found to block virus dissemination from infected mononuclear cells to normal myocytes,and to dicrease titers of virus in cultures.These data suggest that Cox B3 modulation of LFA1 expression on leukocytes might be an important step in virus pathogenesis.