IL-6对急性期川崎病患者外周血淋巴细胞凋亡的影响

The effect of IL-6 on peripheral blood lymphocyte apoptosis in acute kawasaki disease

目的拟通过对急性期川崎病（ＫＤ）患者外周血淋巴细胞凋亡的观察，进一步探讨ＫＤ的免疫发病机理。方法２６例ＫＤ患者和２０名正常儿童外周血单个核细胞（ＰＢＭＣ）经抗－ＣＤ３诱导处理，部分用ＩＬ－６单抗处理，之后培养不同时间（０，１２，２４，４８，７２小时）测定凋亡细胞百分率。结果ＫＤ患者凋亡细胞百分率和ＤＮＡ片段化均较正常对照降低（Ｐ＜０．００１）和延迟。ＰＨＡ刺激ＰＢＭＣ培养上清ＩＬ－６水平较正常对照明显升高（Ｐ＜０．００１）。加抗ＩＬ－６的单抗培养或静脉注射免疫球蛋白可显著降低ＩＬ－６水平，凋亡细胞百分率降低和ＤＮＡ片段化延迟发生逆转。结论ＫＤ患者ＰＢＭＣ存在有凋亡降低或延迟。

For further exploration of the pathogenesis of Kawasaki disease(KD),the cellular morphological change,calculation of percentage of apoptotic cells and assaying DNA fragmentation in peripheral blood mononuclear cells(PBMC) stimulated by anti-CD3 for 0,12,24,48,72h in vitro in 26 KD patients and 20 agematched health children were observed.The results revealed that apoptotic cell percentage and DNA fragmentation were markedly decreased(P<0.001) and delayed(in comparison with those in normal controls),which could reach the state of the control group only by stimulation of antiCD3 for 72h.Remarkably increased production of IL6 in cultured supernatants of PBMC induced by phytohemagglutinin(PHA) from KD patients was found in comparison with those of PBMC from controls(P<0001).Adding antiIL6 McAb to the cultures or using intravenous immunoglobulin(IVIG)in vivo led to significantly decreased production of IL 6(P<0001) and reversed decreased apoptotic cell percentage and delayed DNA fragmentation which were compatible with normal controls.It concluded that peripheral lymphocyte apoptosis was inhibited in KD and overproduced IL6 might be involved in this process.