摘要
目的以研究方法LiPA(Line Probe Assay)分析VacA等位基因的表达,了解幽门螺旋杆菌致病机理的理解。方法从三个不同城市87位进行胃镜检查患者的胃粘膜中培养出幽门螺旋杆菌,提取DNA,用LiPA方法分析VacA等位基因。结果(1)87位患者以s1c(88.5%)和m2a(63.2%)分布为主,未发现s1b和s2;(2)混合菌株感染率为41.4%,远远高于西方国家,其中上海的混合感染率最高(62.5%),与北京(41.0%)和南宁(20.8%)相比具有显著性差异,P<0.01;(3)北京、海、南京三个不同城市s1、m等位基因亚型分布率存在差异;(4)溃疡病和非溃疡病患者m1和m2的分布率没有显著性差异。结论我国幽门螺旋杆菌多重菌株感染率较高,不同的m基因型与消化性溃疡的发生无显著性相关。
Objective： Our aim is to study the distribution of alleles of vacA and the relationship between alleles of vacA and the presence of peptic ulcer. Methods： Helicobacter pylori were cultured from gastric biopsy of patients who were endoscope＆ DNA abstracted from bacteria was analyzed with LiPA after PCR to determine the alleles＇ type of vacA. Results： 1, The prevalence rates of slc （88.5%）m1 （46.0%）and m2a （63.2%）are dominant among the 87 patients; Neither slb nor s2 were detected. 2, Multi-strain infection rate is 41.4%, much higher than those in western countries; compared with Beijing （41.0%）and Guangxi （20.8%）, Shanghai has the highest multi-strain infection rate （62.5%）, P〈0.01.3, The prevalence rate of sl and m alleles among patients from Beijing, Shanghai or Nanjing are different. 4, There is no significance distribution difference of vacA m among patients with peptic ulcer disease or non peptic ulcer disease. Conclusion： The multi-strain infection rate is high in China and the alleles of m are not associated with the clinical outcome of peptic ulcer.
出处
《中国消化内镜》
2008年第11期37-42,共6页
Digestive Disease and Endoscopy
