摘要
目的观察碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)对大鼠脑出血后血肿旁大脑皮质超氧化物歧化酶(superoxide dismutase,SOD)活力、丙二醛(malondialdehyde,MDA)含量和Bax、Bcl-2基因表达的影响。方法采用脑内注射胶原酶建立大鼠脑出血模型,脑出血模型建立成功的大鼠分为脑出血组、生理盐水(normal saline,NS)对照组和bFGF治疗组,每组又分为1d、3d、7d三个时间点。bFGF治疗组按8μg/kg剂量肌内注射,1次/d,各组分别于相应时间点取血肿旁大脑皮质,采用RT-PCR检测凋亡调控基因Bax mRNA、Bcl-2 mRNA的表达,以及SOD活力、MDA含量。结果bFGF治疗组与NS对照组相比,血肿旁大脑皮质SOD活力增高(P<0.05),而MDA含量均下降(P<0.05)。bFGF组血肿旁大脑皮质Bax mRNA表达比NS对照组均明显减少(P<0.05),而Bcl-2 mRNA表达比NS对照组明显增高(P<0.05)。结论bFGF能提高大鼠脑出血后大脑皮质SOD活力,降低MDA含量;提高Bcl-2 mRNA的表达,降低Bax mRNA的表达,从而对脑出血后脑损伤具有保护作用。
Objective To study the effects of basic fibroblast growth factor（bFGF）on the activity of SOD and MDA content and the expression of Bax and Bcl-2 genes in the perihematoma in rat with intracerebral hemorrhage（ICH）. Methods The rat model of experimental ICH was established by injection of collaginase Ⅶ into internal capsule of cerebrum. The activity of SOD and content of MDA in perihematoma were detected,the expression levels of Bax and Bcl-2 genes in perihematoma were detected by RT-PCR. Resuits After bFGF intervention, SOD activities in the around perihematoma tissue of the bFGF group were significantly increased compared with NS control group respectively（P〈0.05） ,but MDA contents were significantly decreased（P〈0.05） ;The expression levels of Bax gene of bFGF group were lower than NS control group（P〈0.05）, the expression levels of Bcl-2 gene of bFGF group were significantly increased（P〈0. 05）. Conclusion bFGF can possess the reliable function of eliminating free radicals, and decrease MDA content and increase the activities of SOD. bFGF can increase the expression of Bcl-2 gene, and reduce the expression of Bax gene.
出处
《中国实用神经疾病杂志》
2008年第12期7-9,共3页
Chinese Journal of Practical Nervous Diseases
基金
广西科学基金(桂科回0575013)
