摘要
建立了尿中Ｄ／ＬＡＭＰ和ＭＡＭＰ光学对映体的ＧＣ和ＧＣ／ＭＳ分析方法．药物与手性衍生化试剂Ｌ－ＴＰＣ反应，转化为相应的非对映体并在普通的毛细管上得到分离．方法简便、快速，回收率大于８０％，最低检出限为２～５ｎｇ／ｍＬ．方法可用于药物立体代谢机理研究，也可在法庭科学中用于判断药物来源及毒作用．
The analytical methods using GC and GC/MS for D/L enantiomers of AMP and MAMP in urine were developed. Using L-TPC as a chiral derivatizing reagent,the drugs were converted to the diastereomer and separated on standard achiral capillary column. The methods given allowed simple and rapid procedures,the recoveries were greater than 80%,and the sensitivity limits were 2～5ng/mL, which makes it possible to study stereospecific mechanism involved in the metabolism and to determine the source of amphetamines as well as its toxic effect in forensic science.
出处
《药物分析杂志》
CAS
CSCD
北大核心
1998年第1期21-25,共5页
Chinese Journal of Pharmaceutical Analysis
关键词
苯丙胺
对映体
尿液
气相色谱
amphetamines,enantiomers,chiral derivatization reagent,GC,GC/MS
