稀土等对霍山石斛组培苗石斛多糖含量的影响

Effects of rare earth and so on on polysaccharides content of Dendrobium huoshanense plantlet in vitro

以霍山石斛试管苗为材料，研究硝酸镧稀土（10～40mg／L）、水杨酸（0．2—5．0mg／L）和L-赖氨酸（O．5～12．5mg／L）对石斛多糖含量的影响，并对石斛多糖提取工艺进行探讨。结果表明：稀土镧和L-赖氧酸对石斛多糖含量具有极显著影响，水杨酸有显著影响，在各自浓度范围内提高石斛多糖含量最适浓度分别为20、2．5和0．2mg／L，与对照相比多糖含量（质量分数）分别增加76．33％、77．11％和23．60％。正交试验表明石斛多糖提取工艺的最佳方案为：100mL的石油醚＋100mL体积分数为80％的乙醇＋40mL的料液。各因素对霍山石斛多糖提取的影响大小顺序依次为：体积分数为80％乙醇量、石油醚量、料滴置．

The effects of rare earth lanthanum （ 10140 mg/L） , salicylic acid （ 0.2--5.0 mg/L） and L - lys （ 0.5-- 12.5 mg/L） on the content of polysaccharides in Dendrobium huoshanense seedling were examined under tissue culture condition. The extraction condition of polysaccharides was determined. The results showed that lanthanum and L - lys had highly significant effects on the polysaccharides content, while salicylic acid had significant effects. The best concentration within each definite concentration range in improving the polysaccharidcs in D. huoshanense was 20mg/L lanthanum nitrate, 2.5 mg/L L - lys and 0.2 mg/L salicylic acid. The content increased 76.33% ,77. 11% and 23.60% respectively compared with the control. The orthogonal test showed that the optimum plan of polysaccharides extraction in D. huoshanense was 100 mL of the content of petroleum ether, 100 mL of 80% using ethanol and 40 mL of the feed. The order of three factors to influence polysaccharides extraction was 80% using ethanol, petroleum ether, material over solvent. The above results could be used as the reference for the development and utilization of D. huoshanense resources.