红菜薹游离小孢子培养技术研究

Study on Isolated Microspore Culture in Purple Flowering Stalk

对影响红菜薹游离小孢子培养的关键因素进行分析。研究结果表明,不同红菜薹材料之间的小孢子胚诱导率差异很大;4℃低温预处理2h对小孢子胚的形成具有促进作用;NLN培养基中添加生长素(NAA)和细胞分裂素(6-BA)对小孢子胚诱导率影响不大,添加浓度过大时诱导率反而降低;培养基中添加活性炭能提高小孢子胚诱导率;子叶型胚在15g/L琼脂的培养基上直接成苗率最高,达56.7%;再生植株在MS和1/2MS+IBA(0.5mg/L)中的生根能力最好,1/2MS生根效果其次。

Several factors affecting isolated microspore culture of Purple flowering stalk were studied in experiments. The results showed that there was a considerable variation in the capability of embryogenesis in different materials. The cool pre-treatmcnt at 4℃ on flower buds for 2 h was propitious to the microspore embryogenesis. The addition of auxins （NAA） and the cytokinins （6-BA） in NLN medium showed no significant effect on the frequency of microspore-induced embryos, but when the concentration was excessive, the frequency decreased. The presence of active carbon could increase the frequency of microspore embryogenesis. The cotyledonary embryos developing into whole plantlet on culture media with 15 g/L agar is the optimum, up to 56.7%. The optimum media for the radication of Purple flowering stalk microspore plantlet was MS and 1/2 MS ＋ IBA （0.5 mg/L）, 1/2 MS is the second.