毛细管区带电泳用于分离分析砷化合物的研究

Studies on Speciation of Arsenious Compounds by Capillary Zone Electrophoresis

采用毛细管区带电泳，以磷酸盐为缓冲溶液分离了5种砷的化合物：As（Ⅲ）、DMA、ANA、MMA和As（Ⅴ）．用紫外吸收法在191nm波长下，对实验条件进行了优化；使用扩展光程毛细管研究了实验方法的重现性；测定了各种砷化合物的峰面积与浓度的关系，在最大进样量时测定了检出限，并将该方法用于合成样品的分析．

Using phosphate as buffer solution, five arsenic species: arsenite (As (Ⅲ)),monomethylarsonic acid (MMA), arsanilic acid (ANA), sodium dimethylarsinate (DMA) and arsenate (As (Ⅴ) ) have been separated by capillary zone electrophoresis. Experiment conditions were optimized when the on-line UV detection was operated at 191 nm. The reproducibility of this method has been studied using extended light length capillary. Relation between signal peak area and sample concentration has been measured, and using the largest injection volume, the detection limit has been determined. Some kind of spiked sample has also been detected.