聚乙烯吡咯烷酮增敏荧光光度法测定生物样品中痕量铅

Fluorophotometric Determination of Trace Lead in Biological Samples by Using the Enhancing Effect of PVP

研究了聚乙烯吡咯烷酮（ＰＶＰ）对桑色素（ｍｏｒｉｎ）和铅（Ⅱ）络合反应的增敏作用，发现ｐＨ３．４３时Ｐｂ－ｍｏｒｉｎ－ＰＶＰ三元体系在４３５ｎｍ激发下，４４４ｎｍ处有强荧光发射，提出了荧光光度法测定痕量Ｐｂ的新方法，其线性范围为０～２４０μｇ／Ｌ，对空白１１次测定ＲＳＤ为３．５％，检出限为２．１×１０－２ｍｇ／Ｌ。对标准人发、潞党参和血清样品的测定，结果满意。

This paper gives a new method for the fluorophotometric determination off trace lead in biological samples .The method is based on that morin can react with lead in acidic medium (pH=3.43) to give an intense fluorescence enhanced by polyvinyl pyrrolidone(PVP) .It can raise the sensitivity of the method.The maximum excitation and emission wavelengths are 435nm and 444nm respectively.The detection limit of Pb(Ⅱ) is 2.1×10 -5 g/L and the linear range is 0～240μg/L.Satisfactory results were obtained in the determination of trace lead in various biological samples.