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采集后6小时与72小时制备的冰冻单采血小板质量研究 被引量:2

A comparative study on qualities of frozen platelets prepared 6 h and 72 h after collection
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摘要 目的通过比较采集后6 h和72 h制备的冰冻单采血小板制品的多项质量参数,为冰冻单采血小板的制备标准提供依据。方法以美国产Trima血细胞分离机配套全密闭7 d保存袋采集的单采血小板,分别于采集后6 h和72 h制备为冰冻血小板制品,1周后复苏留样,分别检测血小板计数(PLT,MPV,PCT,PDW)、血小板粘附性、血小板P选择蛋白、PF3A、PF4、pH值、血块收缩试验(血浆法)。结果采集后6 h和72 h制备的冰冻单采血小板,两者相比差异无统计学意义(t<0.01,P>0.05)。单采血小板在不同保存条件下,PF3A均保持了良好的PF3活性,单采血小板在常规或冰冻保存条件下,PF4及P选择蛋白的表达均明显增加,采集后6 h和72 h制备的冰冻单采血小板相比,差异无统计学意义(t<0.01,P>0.05);单采血小板在常规保存3 d内,粘附功能和血块收缩试验没有明显变化,而冰冻保存的单采血小板这两项指标呈明显减退现象,血小板的活力下降明显。结论以美国产Trima血细胞分离机配套全密闭7 d保存袋采集的单采血小板,分别于采集后6 h和72 h制备的冰冻血小板制品,两者的体外指标分析结果相比差异无显著性,且都能有效的改善和控制急性大出血患者的出血倾向,用于抢救急性大出血患者疗效是可靠的。 Objective To offer evidence for preparation criteria of frozen platelet preparation by means of comparison of a number of quality parameters between the frozen platelets prepared at 6 hour and 72 hour time point after collection. Methods Frozen platelet preparation was made 6 h and 72 h after collection of blood platelets gathered in bags by Trima Blood Corpuscle Machine. A week later, the frozen platelet preparation was dissolved and sampled for detection of PLT, MPV, PCT, PDW, platelet adhesiveness, P selectin, PF3A, PF4, pH value, blood clot contractibility (clot retraction test). Results There were no significant differences in a series of quality parameters between the frozen platelets prepared at 6 hours and 72 hours after collection. Under the different conservation conditions, PF3A kept available activity. When the blood platelets were conserved under the conditions at 22 ℃ or at -80 ℃, the expressions of PF3A and P Selection were increased, there was no difference of PF3A and P-Selection expression between the frozen ptatelets prepared at 6 hours and 72 hours after collection. During three days of routine conservation, no change appeared in the eonglutinate function and clot contractibility; hovever, the above parameters were significantly decreased, and the activity of platelets was markedly delined under congealing. Conclusion There are no significant differences in a series of quality parameters between the frozen platelet preparation prepared with gathered blood by Trima Blood Corpuscle Machine at 6 hours and 72 hours after collection, and both of the frozen plate let preparation can effectively improve or control the hemorrhagic tendency of patients suffered urgent massive hemorrhage.
出处 《国际检验医学杂志》 CAS 2008年第12期1077-1079,共3页 International Journal of Laboratory Medicine
关键词 单采血小板 采集后6小时 72小时 冰冻血小板 质量 Blood platelets gathered by machine 6 hours after collection 72 hours aftercollection Frozen platelets Quality
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