采集后6小时与72小时制备的冰冻单采血小板质量研究

A comparative study on qualities of frozen platelets prepared 6 h and 72 h after collection

目的通过比较采集后6 h和72 h制备的冰冻单采血小板制品的多项质量参数,为冰冻单采血小板的制备标准提供依据。方法以美国产Trima血细胞分离机配套全密闭7 d保存袋采集的单采血小板,分别于采集后6 h和72 h制备为冰冻血小板制品,1周后复苏留样,分别检测血小板计数(PLT,MPV,PCT,PDW)、血小板粘附性、血小板P选择蛋白、PF3A、PF4、pH值、血块收缩试验(血浆法)。结果采集后6 h和72 h制备的冰冻单采血小板,两者相比差异无统计学意义(t<0.01,P>0.05)。单采血小板在不同保存条件下,PF3A均保持了良好的PF3活性,单采血小板在常规或冰冻保存条件下,PF4及P选择蛋白的表达均明显增加,采集后6 h和72 h制备的冰冻单采血小板相比,差异无统计学意义(t<0.01,P>0.05);单采血小板在常规保存3 d内,粘附功能和血块收缩试验没有明显变化,而冰冻保存的单采血小板这两项指标呈明显减退现象,血小板的活力下降明显。结论以美国产Trima血细胞分离机配套全密闭7 d保存袋采集的单采血小板,分别于采集后6 h和72 h制备的冰冻血小板制品,两者的体外指标分析结果相比差异无显著性,且都能有效的改善和控制急性大出血患者的出血倾向,用于抢救急性大出血患者疗效是可靠的。

Objective To offer evidence for preparation criteria of frozen platelet preparation by means of comparison of a number of quality parameters between the frozen platelets prepared at 6 hour and 72 hour time point after collection. Methods Frozen platelet preparation was made 6 h and 72 h after collection of blood platelets gathered in bags by Trima Blood Corpuscle Machine. A week later, the frozen platelet preparation was dissolved and sampled for detection of PLT, MPV, PCT, PDW, platelet adhesiveness, P selectin, PF3A, PF4, pH value, blood clot contractibility （clot retraction test）. Results There were no significant differences in a series of quality parameters between the frozen platelets prepared at 6 hours and 72 hours after collection. Under the different conservation conditions, PF3A kept available activity. When the blood platelets were conserved under the conditions at 22 ℃ or at -80 ℃, the expressions of PF3A and P Selection were increased, there was no difference of PF3A and P-Selection expression between the frozen ptatelets prepared at 6 hours and 72 hours after collection. During three days of routine conservation, no change appeared in the eonglutinate function and clot contractibility; hovever, the above parameters were significantly decreased, and the activity of platelets was markedly delined under congealing. Conclusion There are no significant differences in a series of quality parameters between the frozen platelet preparation prepared with gathered blood by Trima Blood Corpuscle Machine at 6 hours and 72 hours after collection, and both of the frozen plate let preparation can effectively improve or control the hemorrhagic tendency of patients suffered urgent massive hemorrhage.