兴奋剂马钱子碱的电化学氧化及其伏安法测定

On the Electrochemical Oxidation and Voltammetric Determination of Brucine

以玻碳电极为工作电极，硫酸钾为支持电解质，在合适的ｐＨ条件下，通过循环伏安法，首次观测到了马钱子碱的不可逆氧化峰。在浓度为２０×１０－４～４０×１０－３ｍｏｌ／Ｌ范围内其峰电流与浓度呈线性关系，检测限为１０×１０－５ｍｏｌ／Ｌ。在正常人体的尿样中，采用标准加入法进行回收实验，其平均回收率为１００８％。该法简便、快速，用５０×１０－４ｍｏｌ／Ｌ的马钱子碱溶液重复测定１０次，相对标准偏差为１８％。对其反应机理作了初步探讨。

The electrochemical oxidation of the stimulant brucine by cyclic voltammetry was studied with a glassy carbon electrode in a solution containing 5 0×10 -4 mol·L -1 of brucine and 0 5 mol·L -1 of supporting electrolyte K 2SO 4. An irreversible oxidation peak of brucine at 0 96 V was observed in a potential range of +0 60～+1 10 V vs . SCE. The anodic current is linearly proportional to the concentration of brucine in the range of 2 0×10 -4 ～4 0×10 -3 mol·L -1 .The detection limit is 1 0×10 -5 mol·L -1 . Recovery experiments of standard addition in human urine samples showed an average recovery of 100 8 %.The method is simple, rapid and sensitive with a relative standard deviation of 1 8 %( n =10)at 5 0×10 -4 mol·L -1 level. The reaction mechanism is discussed.