大蒜辣素对HepG2肝癌细胞增殖的抑制作用

Investigate the effects of allicin on proliferation and apoptpsis of heptoma cell lines HepG2

目的:研究大蒜辣素(Allicin)对肝癌HepG2细胞增殖的影响。方法:采用高效液相色谱法提纯Allicin,运用细胞计数试剂盒(Cell counting kit-8,CCK-8)研究Allicin对HepG2细胞增殖的影响,运用流式细胞仪研究其对细胞凋亡、DNA代谢的影响,运用Western blot研究其对细胞凋亡基因表达的影响。结果:Allicin经HPLC法提纯后其纯度达到99%;Allicin能抑制HepG2细胞的增殖,导致细胞大量凋亡及死亡,凋亡率达到8.67%±3.2%,死亡率达到70.38%±1.8%;也导致细胞DNA代谢发生紊乱,使大部分细胞处于DNA合成的G0/G1期并使进入S期细胞含量减少,延缓细胞增殖。Western blot实验结果表明,Allicin能导致细胞凋亡相关蛋白大量表达。结论:Allicin能抑制肝癌细胞的增殖,导致细胞大量死亡并引发细胞凋亡,并引起细胞DNA代谢发生紊乱,细胞大部分被阻滞在DNA合成的G0/G1期,Western blot实验结果表明,细胞凋亡基因相关蛋白Bax大量表达,而Bcl-2表达降低。

Objective： To investigate the effects of allicin on proliferation of heptoma cell lines HepG2. Methods： Preparative HPLC was used to purify the allicin. Cell counting kit（CCK-8） was exerted to investigate the proliferation of the HepG2 cell and the flow cytometry was used to detect the apoptosis and mortality rate, finally Western blot method was used to study the expression of apoptosis protein. Results： Experimental results showed that allicin could inhibit the proliferation of cells and cause the HepG2 cells apoptosis and death, the highest ratios of apoptosis and death were 8.67% ± 3.2% and 70.38% ± 1.8%, respectively. The HepG2 cells＇ DNA metabolism was disturbed and most of cells were blocked in G0/G1 period resulting in cells proliferation were blocked. Western blot results showed that the ratio of protein corresponding to apoptosis bax/bcl-2 protein was increased indicating the cells was caused to apoptosis by allicin. Conclusion： Allicin could inhibit the HepG2 cell proliferation and cause cell death and apoptosis. Furthermore, allicin could also cause disturbance of HepG2 cell DNA metabolism disturbance, and the apoptosis protein increase its expression.