摘要
目的：建立高效液相色谱手性添加剂法测定特布他林对映体血药浓度。方法：用Waters Atlantis C18色谱柱（4．6mm×150mm，5μm）；流动相为水-乙腈（95：5），其中含8．4mmol·L^-1 β-CD（作为手性流动相添加剂）、0．05mmol·L^-1醋酸铵且pH3．0；流速为0．6mL·min^-1；荧光检测，Ex=280nm，Em=320nm；进样量5山；柱温10℃。结果：特布他林两对映体在0．25—25mg·L^-1的浓度范围内线性良好。结论：该方法简便、快捷，可用于开展临床研究。
Objective： To develop HPLC method for the determination of terbutaline enantiomers in human plasma. Methods： The HPLC condition was column Waters Atlantis C18 （4.6 mm × 150 mm, 5 μm） , and water-acetonitrile （95：5 ） contaning 8.4 mmol·L^-1 β-CD （used as a chiral mobile phase additive）, 0.05 mmol·L^-1 ammonium acetate with pH 3.0 as the mobile phase with a flow rate at 0.6 mL·min^-1. The chromatography was monitored by fluorescence detection （Ex = 280 nm, Em = 320 nm）; column temperature was 10℃. Results： The linear range were 0.25 -25 mg· L^-1, the recovery was 99.35% - 109.76%, inter-day RSD was less than 4% and intra-day RSD was less than 7%. Conclution： This method is simple and rapid. It provides a suitable method in clinical assay of terbutaline enantiomers.
出处
《药学与临床研究》
2008年第6期460-462,共3页
Pharmaceutical and Clinical Research
