HPLC法测定氯霉素氢化可的松滴耳液中两组分的含量

Determination of Two Components in Chloramphenicol and Hydrocortisone Ear Drops by High Performance Liquid Chromatography

采用反相高效液相色谱法，以醋酸泼尼松为内标，ＵＶ检测波长为２４４ｎｍ，以ＹＷＧ－Ｃ１８为固定相，以甲醇－水（６０∶４０）为流动相，同时测定了氯霉素氢化可的松滴耳液中氯霉素和氢化可的松的含量，并进行了线性范围和回收率测定，平均回收率（ｎ＝６）和相对标准偏差分别为：９９．９１％和０．８６％（氯霉素），９９．５８％和１．３４％（氢化可的松）。

Chloramphenicol and hydrocortisone ear drops are possessed of antiinflammatory and antiallergic functions. It can be used effectively in the treatment of tympanitis. It is composed of chloramphenicol and hydrocortisone. In this paper, we report a reversed phase HPLC method for the determination of chloranphenicol and hydrocortisone in the drops. The operatings conditions were YWG C 18 column(5μm,5mm×150mm) at 30℃, mobile phase of methanol water (60∶40,V/V) and UV detector at 244nm. Prednisone acetate was selected as the internal standard. The linear relationship of calibration curves was good in the range of 40 200 and 8 40 mg/L for chloramphenicol and hydrocortisone, respectively (r=0.999 9). The average recovery and RSD of chloramphenicol and hydrocortisone were 99.91%, 0.86% and 99.58%,1.34%(n=6) respectively. The tolal time for separation and determmination was within 9min. The method was simple, sensitive, rapid and accurate.