刺五加多糖对过氧化氢损伤海马神经元中氧自由基的影响

Effect of Acanthopanacis senticosi polysaccharides on oxygen free radical in hippocampal neures impaired by H_2O_2

目的:研究刺五加多糖(ASPS)对由过氧化氢(H2O2)氧化损伤的大鼠海马神经元内氧自由基的影响。方法:运用海马神经细胞原代培养技术,采用H2O2诱导建立细胞氧化损伤模型。观察细胞形态学变化;甲基四氮唑盐(MTT)法测定细胞活性;免疫组化法鉴定海马神经元,化学比色法测定丙二醛(MDA)、H2O2、羟自由基(ROS)含量及细胞外液中乳酸脱氢酶(LDH)的活性。结果:形态学观察显示,与模型组比较,ASPS组细胞损伤程度明显减轻;ASPS组细胞活性(0.46)比模型组细胞活性(0.36)高(P<0.01);MDA、H2O2、ROS的含量及细胞外液中LDH的活力ASPS组明显低于模型组。结论:ASPS能降低海马神经元中氧自由基的含量,并对海马神经元氧化损伤有保护作用。

OBJECTIVE To study the effect of Acanthopanacis senticosi polysaccharides on oxygen free radical in hippocampal neures impaired by H2O2. METHODS The model was induced by H2O2. The change of cell morphology was observed; the viability of hippocampal neure was detected with MTT method; the hippocampal neure was assessed by immunohistochemical method. The content of MDA , H2O2 ,ROS and the vigour of LDH in extraeellular fluid were determined by chemical colorimetry. RESULTS Compared with model group, the morphology damage of hippocampal neures was slighter than that in model group; The viability of hippocampal neures in ASP groups （0. 46） was higher than that of hippocampal neures in model group （0. 36） （P〈0. 01 ） ; The content of MDA , H2O2, ROS and the vigour of LDH in ASPS groups were significantly lower than those in model group. CONCLUSION ASPS can cut down the content of oxygen free radical antioxidase in hippocampal neures,and protect the hippocampal neures from oxidative damage.