运动性疲劳状态下线粒体膜生物学特征的研究-Ⅲ:递增负荷力竭性运动后大鼠肝线粒体膜NADH-CoQ还原酶及肝组织NAD^(+)的变化

Study of Biological Characteristics of Mitochondrial Membrane during Exercise-induced Fatigue Part Ⅲ:Alterations of Activity of NADH-CoQ Reductase of Hepatic Mitochondrial Membrane and Content of Hepatic NAD+ in Rats

以５０大鼠三级递增负荷跑台跑为力竭性运动模型，分别测定了运动后即刻肝线粒体内膜ＭＭＤＨ－ＣｏＱ还原酶（复合体１）活性的变化；肝组织内ＮＡＤ含量及线粒体膜脂质过氧化水平（ＭＤＡ含量）的变化。发现：一次力竭性运动后，大鼠肝线粒体膜复合体１活性及肝组织当中ＭＤ”含量显著性下降，ＭＤＡ含量显著增加。研究提示，递增负荷力竭性运动中线粒体内膜复合体１损害，肝组织呼吸链底物堆积，线粒体氧利用能力下降，可能是运动性线粒体膜损害及疲劳的重要膜分子机制之一。

Using the SD rats with incremental treadmill running as experimental model, the following alterations inSD rats' liver were examined respectively after the exercise: l) The activity of NADH - CoQ reductase inhepatic mitochondrial membrarane(HMM)(complex I);2).The amount of NAD+ in hepatic tissue;3) Thecontent of lipoperoxide(LPO, e.g. MDA content) in HMM. The results showed that, after exercise, theactivity of NADH-CoQ reductase in HMM decreased significantly by 37.3%, so did the NAD+ in liver tissueby 34% (P<0.05,respectively). While the content of LPO in hepatic mitochondria increased significantly(P<0.01).The.results suggested it might be one of mitochondrial membrane molecular characteristics inexercise-induced fatigue that the HMM complex I damaged and the substrates for respiratory chainaccumulated, and the utilization of oxygen in liver mitochondria decreased during the exhaustive exercise.