白介素-2增强混合骨髓移植后抗白血病效应的实验研究

EXPERIMENTAL STUDY OF IL2 ENHANCING THE ANTILEUKEMIA EFFECT AFTER MIXED BONE MARROW TRANSPLANTATION

目的增强混合骨髓移植（ＭＢＭＴ）后移植物抗白血病（ＧＶＬ）效应，减少ＭＢＭＴ后白血病的复发率。方法采用同基因骨髓混合Ｈ┐２半相合异基因骨髓移植，腹腔短期注射白介素２（ＩＬ┐２），观察了对Ｐ３８８小鼠白血病生存的影响。结果ＭＢＭＴ后白血病小鼠的生存时间显著延长，ＭＢＭＴ后腹腔注射ＩＬ┐２，小鼠的存活时间较不用ＩＬ┐２的小鼠显著延长。结论同基因骨髓混合Ｈ┐２半相合异基因骨髓移植具有显著的ＧＶＬ效应，ＭＢＭＴ后应用ＩＬ┐２能够增强ＧＶＬ效应。这一研究为临床提高混合骨髓移植治疗白血病的疗效。

Objective:To improve the effect of graftversusleukemia (GVL),and to decrease the relapse of leukemia after mixed bone marrow transplantation (MBMT).Methods:Syngeneic (DBA/2) mixed with H2 haploidentical 〔DB2F1 (BALB/c×DBA/2)〕 marrow transplantation model was transplanted to DBA/2 mice with P388 leukemia,the effect of IL2 on enhancing antileukemia was observed in this model.Results:After MBMT DBA/2 mice survived longer than those after autologous BMT.IL2 injection shortly after MBMT could significantly increase the life time of P388 leukemia bearing mice.Conclusions:Syngeneic mixed with H2 haploidentical marrow transplantation had a potential way to increase the GVL effect,which could be enhanced by IL2.