真菌诱导子处理的铁皮石斛原球茎HPLC-MS指纹图谱分析

HPLC-MS Finger Print Analysis of Fungal Elicitor Induced Dendrobium candidum Protocorms

目的比较真菌诱导子处理的铁皮石斛原球茎与对照原球茎、铁皮石斛在化学成分上的差别。方法用高效液相色谱-质谱联用技术,比较诱导子处理的铁皮石斛原球茎、对照原球茎和人工栽培的铁皮石斛的氨性氯仿提取物中各成分的色谱峰面积积分值和相对积分面积。结果诱导子处理的铁皮石斛原球茎和对照原球茎中共有26个相同的化学成分,相对积分面积和分别为98.47%和97.54%,其中有19个成分在诱导子处理原球茎中的色谱峰面积积分值高于对照原球茎,它们中分别有5个和6个特有的成分,其中生物碱类成分分别为3个和1个。诱导子处理的原球茎和铁皮石斛中共有25个相同的化学成分,相对积分面积和分别为95.72%和96.44%,其中有17个成分在人工栽培的铁皮石斛中的色谱峰面积积分值高于诱导子处理的原球茎,有8个成分在诱导子处理的原球茎中的色谱峰面积积分值高于铁皮石斛,这其中有7个是生物碱类的成分。结论真菌诱导子处理后,没有改变原球茎的化学背景,但能提高原球茎中化学成分的含量。人工栽培的铁皮石斛的质量明显优于诱导子处理的原球茎。原球茎中的生物碱类成分对诱导作用较敏感。诱导子处理能促进原球茎中生物碱类成分的产生或特异性积累。

OBJECTIVE To study the difference in chemical composition among fungal elicitor induced （FEI） Dendrobium candidum protocorms, CK protocorms and D. candidum. METHODS The area and the relative area of each chromatographic peak in the ammonic chloroform extracts of above three samples were compared by using high performance liquid chromatography-mass spectrometry. RESULTS The summation of the relative areas of 26 identical compositions took up 98.47% in FEI protocorms and 97.54% in CK, and the peak areas of 19 ones were higher in FEI protocorms than those in CK. There were 5 distinct constituents in FEI protocorms and 6 in CK, respectively. The summation of the relative area of 25 identical constituents took up 95.72% in FEI protocorms and 96. 44% in D. candidum. The peak areas of 17 constituents were higher in the latter than those in the former, and 8 constituents were higher in the former than those in the latter. Among the 8 identical constituents, 7 constituents were alkaloids. CONCLUSION Fungal elicitor could increase the content of chemical compositions in D. candidum protocorms as well as keep its chemical characteristics. The quality of D. candidum was much better than that of protocorms. The alkaloids in protocorms could be induced susceptibly to produce and accumulate.